| Full text | |
| Author(s): |
Campos, Rafael
[1]
;
Monica, Fabiola Z.
[1]
;
Oliveira Justo, Alberto Fernando
[1]
;
Cogo, Jose Carlos
[2]
;
Oliveira, Edvana de Toledo
[3]
;
Moreno, Ronilson Agnaldo
[1]
;
Antunes, Edson
[1]
;
De Nucci, Gilberto
[4, 1]
Total Authors: 8
|
| Affiliation: | [1] Univ Estadual Campinas, UNICAMP, Dept Pharmacol, Fac Med Sci, Campinas, SP - Brazil
[2] Brazil Univ, Fac Biomed Engn, Itaquera, SP - Brazil
[3] Univ Vale Paraiba UNIVAP, Sao Jose Dos Campos - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | PLoS One; v. 13, n. 4 APR 19 2018. |
| Web of Science Citations: | 3 |
| Abstract | |
A tetrodotoxin (TTX)-resistant mechanism is responsible for the electrical field stimulation (EFS)-induced contractions and relaxations of Crotalus durissus terrificus corpora cavernosa. Here it was investigated whether this mechanism also occurs in corpora cavernosa and aortae of the non-venomous snake Pantherophis guttatus corpora cavernosa and aortae. Corpora cavernosa and aortic rings isolated from Pantherophis guttatus snake were mounted in organ bath system for isometric tension recording. EFS-induced contractions in both tissues were performed in the presence and absence of guanethidine (30 mu M), phentolamine (10 mu M) and tetrodotoxin (1 mu M). In another set of experiments, the endothelium was removed from aortic rings and EFS-induced contractions were performed in the denuded rings. Electrical field stimulation-induced contractions were frequency-dependent in Pantherophis guttatus corpora cavernosa and aortic rings. The contractions were significantly reduced in the presence of guanethidine (30 mu M) or phentolamine (10 mu M). Pre-treatment with tetrodotoxin had no effect on the EFS-induced contractions of either corpora cavernosa or aortic rings. Surprisingly, the EFS-induced contractions of aortic rings denuded of endothelium were almost abolished. These results indicate that the TTX-resistant mechanism is present in EFS-induced contractions of Pantherophis guttatus corpora cavernosa and aortae. The experiments performed in the aorta indicate that the endothelium is the main source for the release of catecholamines induced by EFS. (AU) | |
| FAPESP's process: | 11/11828-4 - Pharmacological, eletrophysiological and morphological characterization of a novel TTX-resistant sodium channel coupled to corpus cavernosum of snakes |
| Grantee: | Gilberto de Nucci |
| Support Opportunities: | Research Projects - Thematic Grants |