Chronic intermittent hypoxia induces changes on the expression and activity of neprilysin (EC 3.4.24.11) in the brain of rats

Author(s)

de Oliveira, Renato W. ^{[1, 2]} ; Julian, Guilherme S. ^{[1, 2]} ; Perry, Juliana C. ^{[1, 2]} ; Tufik, Sergio ^{[1, 2]} ; Chagas, Jair R. ^{[1, 2, 3, 4]}

Affiliation

^[1] Univ Fed Sao Paulo UNIFESP, Dept Psicobiol, Sao Paulo, SP - Brazil

^[2] Rua Botucatu 862, 1st Floor, BR-04023062 Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, Dept Biofis, Rua Pedro de Toledo, 669, 7th Floor, BR-04039020 Sao Paulo, SP - Brazil

^[4] Rua Pedro de Toledo 669, BR-04039020 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Neuroscience Letters; v. 678, p. 43-47, JUN 21 2018.
• Web of Science Citations: 0

Abstract

Obstructive sleep apnea (OSA) is a frequent sleeping breathing disorder associated with cognitive impairments. Neprilysin (NEP) is responsible for degrading several substrates related to cognition; however, the effect of chronic intermittent hypoxia (CIH) on NEP is still unknown. This study aimed to evaluate the expression and activity of NEP in cognitive-related brain structures of rats submitted to CIH. Western blot, qRT-PCR and enzyme activity assay, demonstrated that a six-week intermittent hypoxia increased NEP expression and activity, selectively in temporal cortex, but not in the hippocampus and frontal cortex. The increase in NEP activity and expression was reverted followed by two weeks recovery in normoxia. These data show that CIH protocol increases the expression and activity of NEP selectively in the temporal cortex. Additional mechanisms must be investigated to elucidate the effects of CIH in cognition. (AU)

• FAPESP's process: 11/16011-6 - Evaluation of the effect of hypoxia on the expression and activity of neprilysin (EC 3.4.24.11), a protease involved in Alzheimer's Disease
• Grantee: Renato Watanabe de Oliveira
• Support Opportunities: Scholarships in Brazil - Master