DNA Adduct Formation in the Lungs and Brain of Rats Exposed to Low Concentrations of [C-13(2)]-Acetaldehyde

Author(s)

Sanchez, Angelica B. ^{[1, 2, 3]} ; Garcia, Camila C. M. ^{[1, 2, 3]} ; Freitas, Florencio P. ^{[1, 4]} ; Batista, Guilherme L. ^[5] ; Lopes, Fernando S. ^[5] ; Carvalho, Victor H. ^[1] ; Ronsein, Graziella E. ^[1] ; Gutz, Ivano G. R. ^[5] ; Di Mascio, Paolo ^[1] ; Medeiros, Marisa H. G. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508020 Sao Paulo, SP - Brazil

^[2] Univ Fed Ouro Preto, Ctr Res Biol Sci, Ouro Preto - Brazil

^[3] Univ Fed Ouro Preto, Inst Phys & Biol Sci, Dept Biol Sci, Ouro Preto - Brazil

^[4] Helmholtz Zentrum Munchen, Inst Dev Genet, Neuherberg - Germany

^[5] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, BR-05508020 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Chemical Research in Toxicology; v. 31, n. 5, p. 332-339, MAY 2018.
• Web of Science Citations: 3

Abstract

Air pollution is a major environmental risk for human health. Acetaldehyde is present in tobacco smoke and vehicle exhaust. In this study, we show that {[}C-13(2)]-acetaldehyde induces DNA modification with the formation of isotopically labeled 1,N-2-propano-2'-deoxyguanosine adducts in the brain and lungs of rats exposed to concentrations of acetaldehyde found in the atmosphere of megacities. The adduct, with the addition of two molecules of isotopically labeled acetaldehyde {[}C-13(4)]-1,N-2-propano-dGuo, was detected in the lung and brain tissues of exposed rats by micro-HPLC/MS/MS. Structural confirmation of the products was unequivocally performed by nano-LC/ESI+-HRMS3 analyses. DNA modifications induced by acetaldehyde have been regarded as a key factor in the mechanism of mutagenesis and may be involved in the cancer risks associated with air pollution. (AU)

• FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
• Grantee: Ohara Augusto
• Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC