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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Co-Localization of Crotamine with Internal Membranes and Accentuated Accumulation in Tumor Cells

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Author(s):
Mambelli-Lisboa, Nicole Caroline [1, 2] ; Sciani, Juliana Mozer [3, 1] ; Brandao Prieto da Silva, Alvaro Rossan [1, 2] ; Kerkis, Irina [1, 2]
Total Authors: 4
Affiliation:
[1] Butantan Inst, CENTD, BR-05503900 Sao Paulo - Brazil
[2] Butantan Inst, Lab Genet, BR-05503900 Sao Paulo - Brazil
[3] Butantan Inst, Biochem & Biophys Lab, BR-05503900 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Molecules; v. 23, n. 4 APR 2018.
Web of Science Citations: 1
Abstract

Crotamine is a highly cationic; cysteine rich, cross-linked, low molecular mass cell penetrating peptide (CPP) from the venom of the South American rattlesnake. Potential application of crotamine in biomedicine may require its large-scale purification. To overcome difficulties related with the purification of natural crotamine (nCrot) we aimed in the present study to synthesize and characterize a crotamine analog (sCrot) as well investigate its CPP activity. Mass spectrometry analysis demonstrates that sCrot and nCrot have equal molecular mass and biological function-the capacity to induce spastic paralysis in the hind limbs in mice. sCrot CPP activity was evaluated in a wide range of tumor and non-tumor cell tests performed at different time points. We demonstrate that sCrot-Cy3 showed distinct co-localization patterns with intracellular membranes inside the tumor and non-tumor cells. Time-lapse microscopy and quantification of sCrot-Cy3 fluorescence signalss in living tumor versus non-tumor cells revealed a significant statistical difference in the fluorescence intensity observed in tumor cells. These data suggest a possible use of sCrot as a molecular probe for tumor cells, as well as, for the selective delivery of anticancer molecules into these tumors. (AU)

FAPESP's process: 15/50040-4 - Rational approach for searching molecular targets involved in inflammatory events and cell survival
Grantee:Ana Marisa Chudzinski-Tavassi
Support Opportunities: Research Grants - Research Centers in Engineering Program