| Full text | |
| Author(s): |
Mambelli-Lisboa, Nicole Caroline
[1, 2]
;
Sciani, Juliana Mozer
[3, 1]
;
Brandao Prieto da Silva, Alvaro Rossan
[1, 2]
;
Kerkis, Irina
[1, 2]
Total Authors: 4
|
| Affiliation: | [1] Butantan Inst, CENTD, BR-05503900 Sao Paulo - Brazil
[2] Butantan Inst, Lab Genet, BR-05503900 Sao Paulo - Brazil
[3] Butantan Inst, Biochem & Biophys Lab, BR-05503900 Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Molecules; v. 23, n. 4 APR 2018. |
| Web of Science Citations: | 1 |
| Abstract | |
Crotamine is a highly cationic; cysteine rich, cross-linked, low molecular mass cell penetrating peptide (CPP) from the venom of the South American rattlesnake. Potential application of crotamine in biomedicine may require its large-scale purification. To overcome difficulties related with the purification of natural crotamine (nCrot) we aimed in the present study to synthesize and characterize a crotamine analog (sCrot) as well investigate its CPP activity. Mass spectrometry analysis demonstrates that sCrot and nCrot have equal molecular mass and biological function-the capacity to induce spastic paralysis in the hind limbs in mice. sCrot CPP activity was evaluated in a wide range of tumor and non-tumor cell tests performed at different time points. We demonstrate that sCrot-Cy3 showed distinct co-localization patterns with intracellular membranes inside the tumor and non-tumor cells. Time-lapse microscopy and quantification of sCrot-Cy3 fluorescence signalss in living tumor versus non-tumor cells revealed a significant statistical difference in the fluorescence intensity observed in tumor cells. These data suggest a possible use of sCrot as a molecular probe for tumor cells, as well as, for the selective delivery of anticancer molecules into these tumors. (AU) | |
| FAPESP's process: | 15/50040-4 - Rational approach for searching molecular targets involved in inflammatory events and cell survival |
| Grantee: | Ana Marisa Chudzinski-Tavassi |
| Support Opportunities: | Research Grants - Applied Research Centers Program |