Photomodulation multiple sessions as a promising preventive therapy for medication-related osteonecrosis of the jaws after tooth extraction in rats

Author(s):Show less -

Statkievicz, Cristian ^{[1, 2, 3]} ; Toro, Luan Felipe ^{[1, 4]} ; de Mello-Neto, Joao Martins ^{[1, 2, 3]} ; de Sa, Daniela Pereira ^{[1, 2, 3]} ; Casatti, Claudio Aparecido ^{[5, 4]} ; Mardegan Issa, Joao Paulo ^[6] ; Angelo Cintra, Luciano Tavares ^[7] ; de Almeida, Juliano Milanezi ^{[1, 2, 3]} ; Hitomi Nagata, Maria Jose ^{[1, 2, 3]} ; Garcia, Valdir Gouveia ^{[1, 2, 3]} ; Theodoro, Leticia Helena ^{[1, 2, 3]} ; Ervolino, Edilson ^{[1, 5, 4]}

Affiliation

^[1] Sao Paulo State Univ UNESP, Grp Res & Study Laser Dent, Sch Dent Aracatuba, Sao Paulo - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Surg, Sch Dent, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Integrated Clin, Sch Dent, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Inst Biosci, Rua Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP - Brazil

^[5] Sao Paulo State Univ UNESP, Dept Basic Sci, Sch Dent, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[6] Univ Sao Paulo, Dept Morphol Physiol & Basic Pathol, Sch Dent, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[7] Sao Paulo State Univ UNESP, Dept Restorat Dent, Sch Dent, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 184, p. 7-17, JUL 2018.
• Web of Science Citations: 6

Abstract

The aim of this study was to evaluate the effects of photobiomodulation (PBM) with multiple sessions of low-level laser on the alveolar repair process of rats with major risk factors for medication-related osteonecrosis of the jaws (MRONJ). Senile rats received 0.45 mL of vehicle (VEH and VEH-PBM) or 0.45 mL of 100 mu g/kg zoledronate (ZOL and ZOL-PBM) administrated intraperitoneally every two days during seven weeks. After three weeks of initiation of drug treatment the first lower left molar was extracted. No local treatment was performed in VEH and ZOL. VEH-PBM and ZOL-PBM were submitted to laser irradiation (660 +/- 10 nm; 0.035 W; 2.1 J; 60 s) on the extraction site at 0, 2 and 4 days postoperatively. Euthanasia was performed 28 days after tooth extraction. Histological sections of the hemimandible were submitted to histopathological and histomorphometric analysis, as well as to histochemistry for collagen fiber maturation and immunohistochemistry for proinflammatory cytokines. In ZOL, general impairment of tissue repair, areas with osteonecrosis, lower newly formed bone tissue (NFBT), smaller amount of mature collagen fibers and increased immunoreactivity for TNF alpha, IL-1 beta and IL-6 were observed when compared to VEH and VEH-PBM. ZOL-PBM showed significant improvement in some parameters compared to ZOL, such as positive repair tissue, higher NFBT, greater amount of mature collagen fibers, besides TNF alpha and IL-1 beta immunoreactivity decrease. Zoledronate treatment severely compromised the tissue repair process of the tooth extraction site in rats with major risk factors for MRONJ. Based on parameters employed in the present study, PBM in multiple sessions can improve the alveolar repair process, constituting a promising preventive therapy to avoid the onset of post-extraction MRONJ. (AU)

• FAPESP's process: 13/26779-4 - Effect of zoledronate during the alveolar healing process in rats: study involving the major risk factors for developing osteonecrosis of the jaws and assessment of antimicrobial photodynamic therapy (aPDT) as preventive therapy
• Grantee: Edilson Ervolino
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/03067-6 - Expression of teneurins (TEN) and teneurins C-terminal associated peptides (TCAP) in the central nervous system of non-human primates (Cebus apella)
• Grantee: Cláudio Aparecido Casatti
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/02199-1 - Evaluation of the effect of Antimicrobial Photodynamic Therapy (aPDT) during the alveolar healing process in rats that presents the major risk factors for developing osteonecrosis of the jaws associated with the use of bisphosphonates
• Grantee: Cristian Statkievicz
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/14118-6 - Immunohistochemical evaluation of the action of zoledronate during healing process of soft tissue overlying the site of tooth extraction in rats with main risk factors for osteonecrosis of the jaws
• Grantee: João Martins de Mello Neto
• Support Opportunities: Scholarships in Brazil - Scientific Initiation