| Full text | |
| Author(s): |
Total Authors: 4
|
| Affiliation: | [1] Sao Paulo State Univ, UNESP, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil
[2] FACERES Sch Med, Dept Med, Sao Jose Do Rio Preto, SP - Brazil
[3] Univ Fed Sao Paulo, UNIFESP, Dept Morphol & Genet, Sao Paulo, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 8, JUN 22 2018. |
| Web of Science Citations: | 2 |
| Abstract | |
Galectin-1 protein (GAL-1) has important anti-inflammatory properties, but related pharmacologic approaches to effectively treat or prevent renal ischaemia and reperfusion injury are highly limited. Here, we investigated the effect of GAL-1 in a renal ischaemia-reperfusion injury rat model and an in vitro hypoxia-reoxygenation model with a proximal renal tubular epithelial cell line. In vivo, pretreatment with GAL-1 attenuated the renal parameters changed by ischaemia-reperfusion/hypoxiareoxygenation, with recovery of renal function, protecting against influx of leukocytes, cell death and oxidative stress. Ischaemia-reperfusion/hypoxia-reoxygenation was also associated with increased renal endogenous expression of GAL-1 and intercellular adhesion molecule 1 (ICAM-1) plus augmented levels of proinflammatory cytokines IL-1 beta, TNF-alpha and MCP-1 and decreased anti-inflammatory IL-10 in urine, all of which were abrogated by GAL-1 treatment. In vitro studies demonstrated renal tubular epithelial cells as an important source of GAL-lduring hypoxia-reoxygenation and confirmed the protective effects of exogenous GAL-1 through downregulation of proinflammatory cytokine release by proximal renal tubular epithelial cells. Collectively, our findings confirm the important antiinflammatory role of GAL-1 in kidney ischaemia and reperfusion injury and indicate its promising use as a therapeutic approach. (AU) | |
| FAPESP's process: | 16/02012-4 - Evaluation of the immunomodulatory activity of annexin A1 protein in the regulation of inflammatory disorders of the gastrointestinal system: studies in vivo and in vitro experimental models |
| Grantee: | Sonia Maria Oliani |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/09858-3 - Mechanisms of action of the galectin-1 and -3 proteins in the allergic inflammation: Study in in vivo and in vitro experimental models and in allergic patients |
| Grantee: | Cristiane Damas Gil |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/22113-6 - Action mechanism of the anti-inflammatory galectin 1 protein in renal injury induced by the ischemia and reperfusion process: in vivo and in vitro analyses. |
| Grantee: | Carla Patrícia Carlos |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |