| Full text | |
| Author(s): |
Pettian, Mariane Silva
[1]
;
de Guzzi Plepis, Ana Maria
[2, 3]
;
Amaro Martins, Virginia da Conceicao
[2]
;
dos Santos, Geovane Ribeiro
[1]
;
Lopes Pinto, Clovis Antonio
[4, 1]
;
Galdeano, Ewerton Alexandre
[1]
;
Alves Calegari, Amanda Regina
[1]
;
de Moraes, Carlos Alberto
[1]
;
da Cunha, Marcelo Rodrigues
[1, 3]
Total Authors: 9
|
| Affiliation: | [1] Fac Med Jundiai, Dept Morphol & Pathol, Jundiai, SP - Brazil
[2] Univ Sao Paulo, Inst Chem Sao Carlos, Sao Carlos, SP - Brazil
[3] Univ Sao Paulo, Interunit Postgrad Program Bioengn, Sao Carlos, SP - Brazil
[4] AC Camargo Canc Ctr, Dept Anat Pathol, Sao Paulo, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | PLoS One; v. 13, n. 7 JUL 12 2018. |
| Web of Science Citations: | 0 |
| Abstract | |
Polymeric biomaterials composed of extracellular matrix components possess osteoconductive capacity that is essential for bone healing. The presence of collagen and the ability to undergo physicochemical modifications render these materials a suitable alternative in bone regenerative therapies. The objective of this study was to evaluate the osteogenic capacity of collagen-based matrices (native and anionic after alkaline hydrolysis) made from bovine intestinal serosa (MBIS). Twenty-five animals underwent surgery to create a cranial defect to be filled with native and anionic collagen matrixes, mmineralized and non mineralized. The animals were killed painlessly 6 weeks after surgery and samples of the wound area were submitted to routine histology and morphometric analysis. In the surgical area there was new bone formation projecting from the margins to the center of the defect. More marked bone neoformation occurred in the anionic matrices groups in such a way that permitted union of the opposite margins of the bone defect. The newly formed bone matrix exhibited good optical density of type I collagen fibers. Immunoexpression of osteocalcin by osteocytes was observed in the newly formed bone. Morphometric analysis showed a greater bone volume in the groups receiving the anionic matrices compared to the native membranes. Mineralization of the biomaterial did not increase its osteoregenerative capacity. In conclusion, the anionic matrix exhibits osteoregenerative capacity and is suitable for bone reconstruction therapies. (AU) | |
| FAPESP's process: | 12/02767-4 - Usage of mineralized anionic collagen membranes in the reconstruction of bone defects caused in Wistar rats |
| Grantee: | Mariane Silva Pettian |
| Support Opportunities: | Scholarships in Brazil - Master |