Glyphosate effects and phytoalexin accumulation in rust- infected, glyphosate-resi...
| Full text | |
| Author(s): |
Pereira, José Henrique
;
Oliveira, Jaim Simões de
;
Canduri, Fernanda
;
Dias, Marcio Vinicius Bertacine
[4]
;
Palma, Mário Sérgio
;
Basso, Luiz Augusto
;
Azevedo Júnior, Walter Filgueira de
;
Santos, Diógenes Santiago
Total Authors: 8
|
| Document type: | Journal article |
| Source: | Biochemical and Biophysical Research Communications; v. 325, n. 1, p. 10-17, Dec. 2004. |
| Field of knowledge: | Biological Sciences - Biophysics |
| Abstract | |
The crystal structure of shikimate kinase from Mycobacterium tuberculosis (MtSK) complexed with MgADP and shikimic acid (shikimate) has been determined at 2.3 resolution, clearly revealing the amino acid residues involved in shikimate binding. In MtSK, the Glu61 strictly conserved in SK forms a hydrogen bond and salt-bridge with Arg58 and assists in positioning the guanidinium group of Arg58 for shikimate binding. The carboxyl group of shikimate interacts with Arg58, Gly81, and Arg136, and hydroxyl groups with Asp34 and Gly80. The crystal structure of MtSK-MgADP-shikimate will provide crucial information for elucidation of the mechanism of SK-catalyzed reaction and for the development of a new generation of drugs against tuberculosis. (AU) | |
| FAPESP's process: | 01/07532-0 - Structural genomics of cyclin dependent kinases and plant defensive proteinases and their natural inhibitors |
| Grantee: | Walter Filgueira de Azevedo Junior |
| Support Opportunities: | Regular Research Grants |