Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis

Author(s)

de Martini, Cleber Costa ^[1] ; de Andrade, Jessica Thome ^[1] ; Martiniano de Almeida, Stefani Karin ^[1] ; Oliveira Silva, Kathlenn Liezbeth ^[1] ; Eugenio, Flavia de Rezende ^[1] ; Patto dos Santos, Paulo Sergio ^[1] ; Felix de Lima, Valeria Marcal ^[1]

Affiliation

^[1] Univ Estadual Paulista, Dept Clin Care Surg & Anim Reprod, Lab Cellular Immunol, FMVA, UNESP, Fac Vet Med Aracatuba, Rua Clovis Pestana 793, BR-16050680 Sao Paulo - Brazil

• Document type: Journal article
• Source: COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES; v. 57, p. 1-7, APR 2018.
• Web of Science Citations: 1

Abstract

Nitric oxide (NO) is involved in the death of the Leishmania parasite and regulation of apoptosis. We quantified the frequency of cells producing NO and its levels in the peripheral blood mononuclear cells (PBMC), leukocytes from spleen in Visceral Leishmaniasis (VL) symptomatic dogs and correlated NO levels with apoptosis and parasite load in the spleen. The percentage of NO + cells and CD14 + /NO + was higher in PBMC and spleen cells in infected dogs than in controls. The levels of NO + and CD14 + /NO+ cells was higher in PBMC, but lower spleen of dogs infected than compared to control. Late apoptosis rates increased in PBMC and spleen of infected dogs compared to controls, and the NO levels and apoptosis not showed correlation. There was a positive correlation between the percentage of cells producing NO in the spleen and parasite load. The NO participates in the immune response in the canine VL, but it is not apoptosis inducer. (AU)

• FAPESP's process: 13/06684-9 - PD1, apoptosis and arginase in canine visceral leishmaniasis
• Grantee: Valéria Marçal Felix de Lima
• Support Opportunities: Regular Research Grants