| Full text | |
| Author(s): |
Lopes, Magno Alves
[1]
;
Franco, Felipe Oliveira
[1]
;
Henriques, Felipe
[2]
;
Peres, Sidney Barnabe
[3]
;
Batista, Jr., Miguel Luiz
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Mogi das Cruzes, Ctr Integrated Biotechnol, Lab Adipose Tissue Biol, Sao Paulo - Brazil
[2] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA - USA
[3] Univ Estadual Maringa, Dept Physiol Sci, Maringa, Parana - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | HELIYON; v. 4, n. 7 JUL 2018. |
| Web of Science Citations: | 1 |
| Abstract | |
Cancer cachexia (CC) is a multifactorial syndrome with an unknown etiology. The primary symptom is the progressive reduction of the body weight. Recently, down-regulation of adipogenic and lipogenic genes were demonstrated to be early affected during cachexia progression in adipose tissue (AT), resulting in AT remodeling. Thus, this study aimed to evaluate in a co-culture system the influence of the Lewis Lung Carcinoma (LLC) tumor cells (c/c-LLC) in an established preadipocyte cell line 3T3-L1 adipogenic capacity. c/c-LLC in the presence of 3T3-L1 caused a reduction in lipids accumulation, suggesting that secretory tumor cells products may affect adipogenesis. Interestingly, a very early (day 2) downregulation of proliferator-activated receptor gamma (PPAR gamma) and CCAAT/enhancer-binding protein alpha (C/EBP alpha), followed by late genes (day 4 and 8), adiponectin, perilipin, and fatty acid-binding protein 4 (FABP4). Caspase-3 expression was increased on the last day of cell differentiation; it occurred in the expression of pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-alpha). Overall, our results suggest that LLC secretory products impair adipocyte differentiation in a co-culture system and increased apoptosis. In summary, our study has shown the inhibition of the adipogenic process in the 3T3-L1 co-culture system with LLC cells. (AU) | |
| FAPESP's process: | 15/19259-0 - Effects of adipose tissue remodeling during cachexia in gastrointestinal cancer patients: potential role of TLR4 receptor during cachexia-induced browning of WAT |
| Grantee: | Miguel Luiz Batista Junior |
| Support Opportunities: | Regular Research Grants |