Burn injury induces histopathological changes and cell proliferation in liver of rats

Author(s)

Bortolin, Jeferson Andre ^[1] ; Quintana, Hananiah Tardivo ^[1] ; Tome, Tabata de Carvalho ^[1] ; Pidone Ribeiro, Flavia Andressa ^[1] ; Ribeiro, Daniel Araki ^[1] ; de Oliveira, Flavia ^[1]

Affiliation

^[1] Fed Univ Sao Paulo UNIFESP, Dept Biosci, Rua Silva Jardim, 136 Lab 328, BR-11060001 Santos, SP - Brazil

• Document type: Journal article
• Source: WORLD JOURNAL OF HEPATOLOGY; v. 8, n. 6, p. 322-330, FEB 28 2016.
• Web of Science Citations: 2

Abstract

AIM: To investigate effects of severe burn injury (BI) in rat liver through the histopathological and inflammatory markers analysis. METHODS: Forty-two male Wistar rats were distributed into two groups, control (C) and subjected to scald BI (SBI). The animals were euthanized one, four and 14 d post sham or 45% of the total body surface BI. Liver fragments were submitted to histopathological, morphoquantitative (hepatocyte area and cell density), ciclooxigenase-2 (COX-2) immunoexpression, and gene expression {[}real-time polymerase chain reaction for tumor necrosis factor (TNF)-alpha, inducible nitric oxide synthase (iNOS) and caspase-3] methods. RESULTS: Histopathological findings showed inflammatory process in all periods investigated and hepatocyte degeneration added to increased amount of connective tissue 14 d post injury. Hepatocyte area, the density of binucleated hepatocytes and density of sinusoidal cells of SBI groups were increased when compared with control. COX-2 immunoexpression was stronger in SBI groups. No differences were found in TNF-alpha, iNOS and caspase-3 gene expression. CONCLUSION: BI induces histopathological changes, upregulation of COX-2 immunoexpression, and cell proliferation in liver of rats. (AU)

• FAPESP's process: 11/22034-9 - Morphological, genotoxic and molecular effects of scalding thermal injury in gastrocnemius muscle of young rats
• Grantee: Flávia de Oliveira
• Support Opportunities: Regular Research Grants