Influence of the Medium on the Photochemical and Photophysical Properties of [Ru(phen)(2)(pPDIp)](2+)

Author(s):Show less -

de Campos, Isabele Ap. S. ^[1] ; dos Santos, Edjane R. ^[1] ; Sellani, Tarciso Almeida ^[2] ; Herbozo, Carolina C. A. ^[2] ; Rodrigues, Elaine G. ^[2] ; Roveda, Antonio C. ^[3] ; Pazin, Wallance M. ^[4] ; Ito, Amando S. ^[5] ; Santana, Vinicius T. ^[6] ; Nascimento, Otaciro R. ^[6] ; Carlos, Rose M. ^[1]

Affiliation

^[1] Univ Fed Sao Carlos UFSCar, Dept Quim, Rodovia Washington Luis S-N, Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Paulo EPM UNIFESP, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, R Botucatu 862, 88 AndarVila Clementino, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Inst Quim Sao Carlos, Av Trab Sao Carlense, 400Parque Arnold Schimidt, Sao Carlos, SP - Brazil

^[4] Univ Estadual Paulista Julio de Mesquita Filho UN, Dept Fis, Rua Roberto Simonsen 305, Presidente Prudente, SP - Brazil

^[5] Univ Sao Paulo, Dept Fis FFLRP USP, Av Bandeirantes 3900, Ribeirao Preto, SP - Brazil

^[6] Univ Sao Paulo, Inst Fis Sao Carlos, Av Trab Sao Carlense, 400 Parque Arnold Schimidt, Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: CHEMPHOTOCHEM; v. 2, n. 8, p. 757-764, AUG 2018.
• Web of Science Citations: 0

Abstract

This study reports the influence of the medium on the aggregation processes of the complex {[}Ru(phen)(2)(pPDIp)](2+)(phen=1,10-phenanthroline; pPDIp=a bis-phenanthroline-substituted perylene diimide)and its consequences for the photochemical and photophysical properties of the system. Photolysis leads, initially, to emission of both chromophores: Triplet metal-to-ligand charge transfer ((MLCT)-M-3{*}; Ru, dphen, {*}) and singlet intraligand charge transfer ((ILCT)-I-1{*}; pPDIp, {*}). However, the medium induces increased - intermolecular interactions that lead to the generation of a pPDIp(.-)moietyin DMSO, pPDIp(.-) (518nm) and pPDIp(.-) and pPDIp(2-)(420nm) in the cell culture medium RPMI, and (3{*})pPDIp in buffer (pH7.4) and starch films. The complex activates singlet oxygen in buffer solution without oxygen bubbling and under green light irradiation (dose=0.41J/cm(2); {[}O-1(2)]=0.03-0.47molL(-1) for {[}Ru(phen)(2)(pPDIp)](2+)=10-150molL(-1)). No cytotoxic effects on B16F10-Nex2 murine melanoma cell viability were observed up to a concentration of 10molL(-1) of complex. However, under green LED illumination (dose=0.41J/cm(2)) the complex exhibits a strong photocytotoxic effect, displaying IC50=1.2molL(-1). (AU)

• FAPESP's process: 17/00839-1 - Analysis of the structure-toxicity relationship of b-amyloid peptides and neuroprotective effect of luminescent Ru (II) complexes
• Grantee: Rose Maria Carlos
• Support type: Regular Research Grants
• FAPESP's process: 16/09633-4 - Mechanism of action of the bioactive compound Artepillin C in membrane biomimetic systems and cytoplasmic membrane of tumor cells
• Grantee: Wallance Moreira Pazin
• Support type: Scholarships in Brazil - Post-Doctorate