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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Lidocaine and Pinacidil Added to Blood versus Crystalloid Cardioplegic Solutions: Study in Isolated Hearts

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Author(s):
do Carmo, Helison Pereira [1] ; Reichert, Karla [1] ; de Carvalho, Daniela Diogenes [1] ; Silveira-Filho, Lindemberg da Mota [1] ; Vilarinho, Karlos [1] ; Oliveira, Pedro [1] ; Petrucci, Orlando [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Revista Brasileira de Cirurgia Cardiovascular; v. 33, n. 3, p. 211-216, 2018.
Web of Science Citations: 1
Abstract

Abstract Objective: The present study aimed the functional recovery evaluation after long term of cardiac arrest induced by Custodiol (crystalloid-based) versus del Nido (blood-based) solutions, both added lidocaine and pinacidil as cardioplegic agents. Experiments were performed in isolated rat heart perfusion models. Methods: Male rat heart perfusions, according to Langendorff technique, were induced to cause 3 hours of cardiac arrest with a single dose. The hearts were assigned to one of the following three groups: (I) control; (II) Custodiol-LP; and (III) del Nido-LP. They were evaluated after ischemia throughout 90 minutes of reperfusion. Left ventricular contractility function was reported as percentage of recovery, expressed by developed pressure, maximum dP/dt, minimum dP/dt, and rate pressure product variables. In addition, coronary resistance and myocardial injury marker by alpha-fodrin degradation were also evaluated. Results: At 90 minutes of reperfusion, both solutions had superior left ventricular contractile recovery function than the control group. Del Nido-LP was superior to Custodiol-LP in maximum dP/dt (46%±8 vs. 67%±7, P<0.05) and minimum dP/dt (31%±4 vs. 51%±9, P<0.05) variables. Coronary resistance was lower in del Nido-LP group than in Custodiol-LP (395%±50 vs. 307%±13, P<0.05), as well as alpha-fodrin degradation, with lower levels in del Nido-LP group (P<0.05). Conclusion: Del Nido-LP cardioplegia showed higher functional recovery after 3 hours of ischemia. The analysis of alpha-fodrin degradation showed del Nido-LP solution provided greater protection against myocardial ischemia and reperfusion (IR) in this experimental model. (AU)

FAPESP's process: 12/09130-1 - Development of cardioplegic solution for heart transplant and conventional open-heart surgeries.
Grantee:Orlando Petrucci Jr
Support Opportunities: Regular Research Grants