Effect of phospholipase A(2) inhibitors during infection caused by Leishmania (Leishmania) amazonensis

Author(s)

Bordon, Maria L. A. C. ^{[1, 2]} ; Laurenti, Marcia D. ^[1] ; Ribeiro, Susan Pereira ^[3] ; Toyama, Marcos H. ^[2] ; Toyama, Daniela De O. ^[4] ; Passero, Luiz Felipe D. ^[2]

Affiliation

^[1] Univ Sao Paulo, Sch Med, Lab Pathol Infect Dis LIM 50, Ave Dr Arnaldo 455, BR-01246903 Sao Paulo, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Biosci, Praca Infante Dom Henrique S-N, BR-11330900 Sao Vicente, SP - Brazil

^[3] Case Western Reserve Univ, Dept Pathol, 2103 Cornell Rd, Room 5503, Cleveland, OH 44106 - USA

^[4] Camilo Castelo Branco Univ Unicastelo, Sch Dent, Rua Carolina Fonseca 584, BR-08230030 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 24, AUG 27 2018.
• Web of Science Citations: 2

Abstract

Background: Lipid metabolites play an important role in parasite differentiation and virulence. Studies have revealed that Leishmania sp. uses prostaglandins to evade innate barriers, thus enabling the parasites to survive inside immune cells. Despite the role of the enzyme Phospholipase A(2) (PLA(2)) in prostaglandins production, few studies have investigated the role of parasite PLA(2) during the interaction between L. (L.) amazonensis and the host (in vitro and in vivo) immune cells. Methods: In the present work, the leishmanicidal effect of PLA(2) inhibitors, methyl arachidonyl fluorophosphonate (MAFP), bromoenol lactone (BEL) and aristolochic acid (AA) were investigated in vitro (promastigote and intracellular amastigote forms of L. (L.) amazonensis) and during in vivo infection using BALB/c mice. Results: The aforementioned inhibitors were deleterious to promastigote and amastigote forms of the L. (L.) amazonensis and were non-toxic to peritoneal macrophages from BALB/c mice. L. (L.) amazonensis-infected BALB/c mice treated with the inhibitor BEL presented decreased lesion size and skin parasitism; however, BEL treatment induced hepatotoxicity in BALB/c mice. Conclusions: Results presented herein suggested that PLA(2) inhibitors altered L. (L.) amazonensis viability. In spite of liver toxicity, treatment with BEL was the most selective compound in vitro, as well in vivo, resulting in lower skin parasitism in the infected mice. These findings corroborate the role of PLA(2) in parasite virulence and maintenance in vertebrate hosts, and suggest that molecules structurally related to BEL should be considered when planning compounds against Leishmania sp. (AU)

• FAPESP's process: 16/00468-0 - Use of drug repurposing and natural product bioprospection to characterize compounds with in vitro and in vivo leishmanicidal action
• Grantee: Luiz Felipe Domingues Passero
• Support Opportunities: Regular Research Grants