Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development of a qPCR for the detection of infectious laryngotracheitis virus (ILTV) based on the gE gene

Full text
Author(s):
Parra, Silvana Santander [1] ; Nunez, Luis [1] ; Buim, Marcos R. [2] ; Astolfi-Ferreira, Claudete S. [1] ; Piantino Ferreira, Antonio J. [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Sch Vet Med, Dept Pathol, Ave Prof Dr Orlando M Paiva 87, BR-05508270 Sao Paulo, SP - Brazil
[2] Inst Biol, Lab Avian Dis, Bastos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BRITISH POULTRY SCIENCE; v. 59, n. 4, p. 402-407, 2018.
Web of Science Citations: 0
Abstract

1. Infectious laryngotracheitis is a respiratory disease that affects the poultry industry worldwide. It is common in flocks with high-bird density, causing major economic losses.2. In this study, a SYBR (R) FAST polymerase chain reaction (PCR) double-strand DNA intercalating agent assay was performed for the detection of infectious laryngotracheitis virus (ILTV) in clinical samples in comparison with a conventional nested-PCR, both based on the glycoprotein E encoding gene. This assay amplified 56bp and was capable of detecting 1(9) to 1 copies of virus.3. In total, 164 clinical samples were obtained from birds with respiratory problems from the period of 2009-2016. In the nested-PCR, there were 45.12% positive samples and 54.88% negative samples, while in the real-time PCR (qPCR), there were 81.1% positive samples and 18.9% negative samples.4. In conclusion, qPCR from the DNA double-strand intercalating agent SYBR (R) GREEN FAST was useful for the diagnosis of ILTV because it detected samples that were negative in nested-PCR. This assay has advantages, such as a shortened processing-time, and no need for post-amplification processing (electrophoresis) with additional reagents, such as MgCl2 and agarose. Hence, qPCR proved to be useful, rapid and low cost for use with clinical samples. (AU)

FAPESP's process: 12/20912-1 - Prevalence, molecular characterization and interactions of avian mycoplasmas and laryngotracheitis infectious virus in laying hens of Bastos' region
Grantee:Marcos Roberto Buim
Support Opportunities: Regular Research Grants