Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)

Author(s)

Castro, Talita ^[1] ; Sabalza, Maite ^[2] ; Barber, Cheryl ^[2] ; Abrams, William ^[2] ; Da Costa, Antonio Charlys ^[3] ; De Padua Milagres, Flavio Augusto ^{[4, 5]} ; Braz-Silva, Paulo Henrique ^{[1, 3]} ; Malamud, Daniel ^[2] ; Gallottini, Marina ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Dent, Stomatol Dept, Sao Paulo, SP - Brazil

^[2] NYU, Coll Dent, Dept Basic Sci & Craniofacial Biol, New York, NY - USA

^[3] Univ Sao Paulo, Inst Trop Med, Lab Med Res, Sao Paulo, SP - Brazil

^[4] Secretary Hlth Tocantins, Epidemiol Surveillance & Infect Dis, Palmas, TO - Brazil

^[5] Fed Univ Tocantins, Palmas, TO - Brazil

• Document type: Journal article
• Source: JOURNAL OF ORAL MICROBIOLOGY; v. 10, n. 1 SEP 3 2018.
• Web of Science Citations: 1

Abstract

Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplification (LAMP) is a rapid assay that detects nucleic acids, including ZIKV RNA. Aim: The aim of this study was to evaluate the efficacy of saliva and urine to diagnose ZIKV infection in subjects during the acute phase, through ZIKV RNA detection by LAMP. Method: A total of 131 samples (68 saliva and 63 urine samples) from 69 subjects in the acute phase of ZIKV infection, and confirmed positive for ZIKV by blood analysis through real time-PCR, were collected and analyzed by Reverse Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP). Results: From the 68 saliva samples, 45 (66.2%) were positive for ZIKV with an average time to positivity (Tp) of 13.5min, and from the 63 urine samples, 25 (39.7%) were positive with the average Tp of 15.8min. Saliva detected more samples (p=0.0042) and had faster Tp (p=0.0176) as compared with urine. Conclusion: Saliva proved to be a feasible alternative to diagnose ZIKV infection during the acute phase by LAMP. (AU)

• FAPESP's process: 17/00021-9 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Antonio Charlys da Costa
• Support type: Scholarships in Brazil - Post-Doctorate