Unveiling the complete genome sequence of clerodendrum chlorotic spot virus, a putative dichorhavirus infecting ornamental plants

Author(s)

Ramos-Gonzalez, Pedro Luis ^[1] ; Chabi-Jesus, Camila ^{[2, 1]} ; Banguela-Castillo, Alexander ^[1] ; Tassi, Aline Daniele ^[2] ; Rodrigues, Mariane da Costa ^{[1, 3]} ; Kitajima, Elliot Watanabe ^[2] ; Harakava, Ricardo ^[1] ; Freitas-Astua, Juliana ^{[4, 1]}

Affiliation

^[1] Inst Biol, Lab Bioquim Fitopatol, BR-04014002 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil

^[3] Univ Anhembi Morumbi UAM, BR-03164000 Sao Paulo, SP - Brazil

^[4] Embrapa Mandioca & Fruticultura, BR-44380000 Cruz Das Almas, BA - Brazil

• Document type: Journal article
• Source: ARCHIVES OF VIROLOGY; v. 163, n. 9, p. 2519-2524, SEP 2018.
• Web of Science Citations: 2

Abstract

The genus Dichorhavirus includes plant-infecting rhabdoviruses with bisegmented genomes that are horizontally transmitted by false spider mites of the genus Brevipalpus. The complete genome sequences of three isolates of the putative dichorhavirus clerodendrum chlorotic spot virus were determined using next-generation sequencing (Illumina) and traditional RT-PCR. Their genome organization, sequence similarity and phylogenetic relationship to other viruses, and transmissibility by Brevipalpus yothersi mites support the assignment of these viruses to a new species of dichorhavirus, as suggested previously. New data are discussed stressing the reliability of the current rules for species demarcation and taxonomic status criteria within the genus Dichorhavirus. (AU)

• FAPESP's process: 13/25713-0 - Evaluation of the morphological and genomic diversity in different populations of Brevipalpus (Acari: Tenuipalpidae) species, vector of plant viruses, and their competence for virus transmission
• Grantee: Aline Daniele Tassi
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/08458-9 - Plant viruses transmitted by Brevipalpus mites (Acari: Tenuipalpidae) - BTV: survey, identification, molecular characterization, phylogeny; virus/vector/host relationhip; biology, taxonomy and management of the mite
• Grantee: Elliot Watanabe Kitajima
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 16/01960-6 - Molecular characterization, phylogeny and evolución of Brevipalpus-transmitted viruses in Brazil
• Grantee: Camila Chabi de Jesus
• Support type: Scholarships in Brazil - Doctorate