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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Role of hypothetical protein YicS in the pathogenicity of Avian Pathogenic Escherichia coli in vivo and in vitro

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Author(s):
Verma, Renu [1] ; Galvao Rojas, Thais Cabrera [1] ; Maluta, Renato Pariz [1] ; Leite, Janaina Luisa [1] ; Nakazato, Gerson [2] ; de Silveira, Wanderley Dias [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Genet Evolut & Bioagents, POB 6109, BR-13083875 Campinas, SP - Brazil
[2] Univ Londrina, UEL, Inst Biol, Dept Microbiol, Biol Sci Ctr, BR-86055990 Londrina, PR - Brazil
Total Affiliations: 2
Document type: Journal article
Source: MICROBIOLOGICAL RESEARCH; v. 214, p. 28-36, 2018.
Web of Science Citations: 1
Abstract

Avian Pathogenic Escherichia coil (APEC) strains belong to the extra-intestinal pathogenic group of E. coli (ExPEC) that causes colibacillosis in poultry. A variety of putative virulence factors of APEC are recognized as potent causes of pathogenicity, the mechanisms underlying their pathogenicity are still not fully understood. The role of yicS in the virulence of pathogenic E. coil is still unclear. Thus, yicS may be related to biofilm formation, which in some bacteria plays a role in pathogenicity. Therefore, the fact that this gene appears to be under positive selection pressure suggests that yicS may be associated with the pathogenicity of APEC. To better understand the role of yicS protein in APEC biological characteristics and pathogenicity, we deleted yicS in an APEC Swollen Head Syndrome strain (APEC strain SCI-07) and studied its effects by comparing wild type and isogenic mutants through comprehensive in vitro and in vivo assays. We demonstrated that yicS plays a role in pathogenicity of APEC. We suggest that the yicS gene, which encodes an exporter protein, has a significant role in biofilm formation, motility, invasion of CEC-32 and Hep-2 cells and APEC pathogenicity in a day-old chick model. (AU)

FAPESP's process: 15/03860-6 - Analysis of gene expression and phenotipic characteristics of avian pathogenic Escherichia coli strains(APEC) mutants for lysin descarboxilase operon (LDC-)
Grantee:Wanderley Dias da Silveira
Support type: Regular Research Grants
FAPESP's process: 12/05073-3 - MOLECULAR CHARACTERIZATION OF AVIAN PATHOGENIC (APEC) AND HUMAN UROPATHOGENIC (UPEC) ESCHERICHIA COLI LINEAGES
Grantee:Renato Pariz Maluta
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/09167-5 - Site-directed mutagenesis of a new autotransporter in an avian pathogenic Escherichia coli strain (APEC) and evaluation of the contribution of this gene to pathogenesis
Grantee:Thaís Cabrera Galvão Rojas
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/04860-7 - Study and molecular characterization of Uropathogenics Escherichia coli strains and its potential as pathogenic strains for poultry (APEC)
Grantee:Janaína Luisa Leite Garbim
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 12/23289-3 - Deletion of putative invasin and DnaK genes from avian pathogenic Escherichia coli (APEC) strain SCI07 to study their role in pathogenicity and obtain attenuated vaccine strains
Grantee:Renu Verma
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/09655-7 - In vivo analyses of genic expression and mutation in quorum sensing gene AI-2 of an avian Pathogenic Escherichia coli strain
Grantee:Wanderley Dias da Silveira
Support type: Regular Research Grants