| Full text | |
| Author(s): Show less - |
Rogeri, Caroline Domingues
[1]
;
Santejo Silveira, Henrique Cesar
[1]
;
Causin, Rhafaela Lima
[1]
;
Villa, Luisa Lina
[2, 3]
;
Stein, Maira Degiovani
[4]
;
de Carvalho, Ana Carolina
[1]
;
Rebolho Batista Arantes, Lidia Maria
[1]
;
Scapulatempo-Neto, Cristovam
[5]
;
Possati-Resende, Julio Cesar
[6]
;
Antoniazzi, Marcio
[6]
;
Longatto-Filho, Adhemar
[7, 1, 8, 9]
;
Tavares Guerreiro Fregnani, Jose Humberto
[10, 1]
Total Authors: 12
|
| Affiliation: | [1] Barretos Canc Hosp, Mol Oncol Res Ctr, Av Antenor Duarte Vilela 1331, BR-14784400 Barretos - Brazil
[2] Univ Sao Paulo, Sch Med, Dept Radiol & Oncol, Sao Paulo - Brazil
[3] ICESP Canc Inst State Sao Paulo, Sao Paulo - Brazil
[4] Barretos Canc Hosp, Dept Pathol, Barretos - Brazil
[5] DASA Diagnost Amer, Barueri - Brazil
[6] Barretos Canc Hosp, Dept Prevent, Barretos - Brazil
[7] ICVS 3Bs PT Govt Associate Lab, Braga - Portugal
[8] Minho Univ, Sch Med, Life & Hlth Sci Res Inst, ICVS, Braga - Portugal
[9] Univ Sao Paulo, Sch Med, Dept Pathol, LIM14, Sao Paulo - Brazil
[10] AC Camargo Canc Ctr, Sao Paulo - Brazil
Total Affiliations: 10
|
| Document type: | Journal article |
| Source: | GYNECOLOGIC ONCOLOGY; v. 150, n. 3, p. 545-551, SEP 2018. |
| Web of Science Citations: | 5 |
| Abstract | |
Objectives. The methylation profile of genes in precursor lesions in cervical cancer was characterized to improve screening techniques for high-grade intraepithelial neoplasia. Methods. A total of 447 cervical cytology samples obtained from women who underwent colposcopy were examined. The cases were distributed as follows: (1) cervices without cervical intraepithelial neoplasia (CIN; n = 152); (2) cervices with a CIN grade of 1 (CIN 1; n = 147); and (3) cervices with a ON grade of 2 or 3 (CIN 2/3; n = 148). The methylation pattern for a panel of 15 genes was analysed by quantitative methylation-specific PCR (qMSP) and compared between the groups (<= CIN 1 vs. CIN 2+). Results. In the validation set, seven genes presented significantly different methylation profiles according to diagnosis, namely, DAPK1 (p = 0.001), EPB41L3 (p = 0.001), HIC1 (p = 0.028), hsa-miR-124-2 (p = 0.001), LMX1A (p = 0.001), SOX1 (p = 0.001), and TERT (p = 0.001). Six genes showed a significant increase in the frequency of methylation in the presence of hr-HPV, namely, DAPK1 (p = 0.001), EPB41L3 (p = 0.001), hsa-miR-124-2 (p = 0.001), LMXIA (p = 0.001), SOX1 (p = 0.001), and TERT (p = 0.001). The methylation of the hsa-miR-124 gene showed sensitivity and specificity (86.7% and 613%, respectively) similar to that of the HPV test (913% and 50.0%, respectively). The independent factors associated with the diagnosis of CIN 2+ and the methylation of the hsa-miR-124-2 (OR = 5.1), SOX1 (OR = 2.8), TERT (OR = 2.2), and LMXIA (OR = 2.0) genes were a positive test for hr-HPV (odds ratio {[}OR] = 5.5). Conclusions. Hypermethylation of the hsa-miR-124-2, SOX1, TERT, and LMXIA genes may be a promising bio-marker for precursor lesions in cervical cancer regardless of the hr-HPV status. (C) 2018 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 12/51221-4 - Analysis of sensitivity and specificity of four algorithms for cervical cancer screening combining cervical cytology and Human Papillomavirus molecular test (HPV) |
| Grantee: | Adhemar Longatto Filho |
| Support Opportunities: | Research Grants - Research in Public Policies for the National Health Care System (PP-SUS) |
| FAPESP's process: | 14/50014-0 - Genes methylation profile in uterine cervical cancer precursor lesions |
| Grantee: | José Humberto Tavares Guerreiro Fregnani |
| Support Opportunities: | Research Grants - Research in Public Policies for the National Health Care System (PP-SUS) |