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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of a novel Drosophila melanogaster cis-regulatory module that drives gene expression to the larval tracheal system and adult thoracic musculature

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Author(s):
Wilfredo Cachay Wester, Jorge Victor [1] ; Couto Lima, Carlos Antonio [2] ; Rosa Machado, Maiaro Cabral [3] ; Zampar, Patricia Vieira [4] ; Tavares, Simone Sakagute [4] ; Monesi, Nadia [4]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Programa Posgrad Biol Celular & Mol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Fis & Quim, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: GENESIS; v. 56, n. 8 AUG 2018.
Web of Science Citations: 0
Abstract

In a previous bioinformatics analysis we identified 10 conserved Drosophila melanogaster sequences that reside upstream from protein coding genes (CGs). Here we characterize one of these genomic regions, which constitutes a Drosophila melanogaster cis-regulatory module (CRM) that we denominate TT-CRM. The TT-CRM is 646bp long and is located in one of the introns of CG32239 and resides about 3,500bp upstream of CG13711 and about 620bp upstream of CG12493. Analysis of 646bp-lacZ lines revealed that TT-CRM drives gene expression not only to the larval, prepupal, and pupal tracheal system but also to the adult dorsal longitudinal muscles. The patterns of mRNA expression of the transgene and of the CGs that lie in the vicinity of TT-CRM were investigated both in dissected trachea and in adult thoraces. Through RT-qPCR we observed that in the tracheal system the pattern of expression of 646bp-lacZ is similar to the pattern of expression of CG32239 and CG13711, whereas in the thoracic muscles 646bp-lacZ expression accompanies the expression of CG12493. Together, these results suggest new functions for two previously characterized D. melanogaster genes and also contribute to the initial characterization of a novel CRM that drives a dynamic pattern of expression throughout development. (AU)

FAPESP's process: 08/03476-8 - Functional validation of potential ring gland enhancers identified in the Drosophila melanogaster genome
Grantee:Patricia Vieira Zampar
Support Opportunities: Scholarships in Brazil - Scientific Initiation