Evaluation of cytotoxicity features of antimicrobial peptides with potential to control bacterial diseases of citrus

Author(s)

Inui Kishi, Rosangela Naomi ^{[1, 2, 3]} ; Stach-Machado, Dagmar ^[1] ; Singulani, Junya de lacorte ^[4] ; dos Santos, Claudia Tavares ^[4] ; Fusco-Almeida, Ana Marisa ^[4] ; Cilli, Eduardo Maffud ^[5] ; Freitas-Astua, Juliana ^[6] ; Picchi, Simone Cristina ^[2] ; Machado, Marcos Antonio ^[2]

Affiliation

^[1] Univ Estadual Campinas, Inst Biol, Sao Paulo - Brazil

^[2] Inst Agron, Ctr Citricultura Sylvio Moreira, Sao Paulo - Brazil

^[3] Fundo Def Citricultura, FUNDECITRUS, Sao Paulo - Brazil

^[4] Univ Estadual Paulista, Fac Ciencias Farmaceut, Sao Paulo - Brazil

^[5] Univ Estadual Paulista, Inst Quim Araraquara, Dept Bioquim & Tecnol Quim, Sao Paulo - Brazil

^[6] Embrapa Cassava & Fruits, Cruz Das Almas, BA - Brazil

• Document type: Journal article
• Source: PLoS One; v. 13, n. 9 SEP 7 2018.
• Web of Science Citations: 3

Abstract

Antimicrobial peptides (AMPs) can be found in various organisms, and could be considered an alternative for pesticides used to control plant pathogens, including those affecting citrus. Brazil is the largest producer and exporter of frozen concentrated orange juice in the world. However, the citrus industry has been affected by several diseases such as citrus canker and huanglongbing (HLB), caused by the bacteria Xanthomonas citri subsp. citri (X. citri) and Candidatus Liberibacter asiaticus (CaLas), respectively. In order to control these pathogens, putative AMPs were prospected in databases containing citrus sequences. Furthermore, AMPs already reported in the literature were also used for in vitro and in vivo assays against X. citri. Since CaLas cannot be cultivated in vitro, surrogates as Sinorhizobium meliloti and Agrobacterium tumefaciens were used. This study reports the evaluation of six AMPs obtained from different sources, two of them from Citrus spp. (citrus-amp1 and citrus-amp2), three from amphibians (Hylin-a1, K-0-W-6-Hy-a1 and Ocellatin 4-analogue) and one from porcine (Tritrpticin). Peptides K-0-W-6-Hy-a1, Ocellatin 4-analogue, and citrus-amp1 showed bactericidal activity against X. citri and S. meliloti and bacteriostatic effect on A. tumefaciens. These results were confirmed for X. citri in planta. In addition cytotoxicity evaluations of these molecules were performed. The AMPs that showed the lowest hemolytic activities were Triptrpticin, citrus-amp1 and citrus-amp2. Citrus-amp1 and citrus-amp2 not presented toxicity in experiments using in vivo model, G. mellonella and U87 MG cells. To verify the interaction of these AMPs with bacteria and erythrocyte cell membranes, vesicles mimicking these cells were built. Citrus-amp1 and Tritrpticin exhibited higher affinity to bacterial membranes, while Ocellatin 4-analogue and Hylin-a1 showed higher affinity to erythrocyte membranes; exclude their use in citrus. This work demonstrates an essential alternative, trough AMPs obtained from Citrus spp., which can be feasibly used to control bacterial pathogens. (AU)

• FAPESP's process: 08/52691-9 - Management of citrus leprosis
• Grantee: Elliot Watanabe Kitajima
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 12/15346-7 - Antimicrobial peptides: dimerization effects and encapsulation in NRLb biomembrane
• Grantee: Eduardo Maffud Cilli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/11072-4 - EXPLORATION AND ANALYSIS OF ANTIMICROBIAL PEPTIDES ACTIVITY TO CONTROL PATHOGENS OF CITRUS
• Grantee: Rosangela Naomi Inui Kishi
• Support Opportunities: Scholarships in Brazil - Doctorate