Modulation of epigenetic factors during the early stages of HIV-1 infection in CD4(+) T cells in vitro

Author(s)

Nunes, Jorge Meneses ^[1] ; Furtado, Maria Nadiege ^[2] ; de Morais Nunes, Edsel Renata ^[2] ; Araripe Sucupira, Maria Cecilia ^[2] ; Diaz, Ricardo Sobhie ^[2] ; Ramos Janini, Luiz Mario ^{[1, 2]}

Affiliation

^[1] Univ Fed Sao Paulo, Lab Retrovirol, Dept Microbiol Immunol & Parasitol, Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Lab Retrovirol, Discipline Infect Dis, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: VIROLOGY; v. 523, p. 41-51, OCT 2018.
• Web of Science Citations: 1

Abstract

Several studies have related epigenetic mechanisms to HIV-1 latency. However, the epigenetic modifications of the host cell genome involved in the early stages of HIV-1 infection remain unclear. This study aimed to investigate epigenetic factors that are regulated at the beginning of HIV-1 infection in activated and resting CD4(+) T cells. We analyzed the gene expression of 84 epigenetic targets, global DNA methylation, and HIV-1 replication kinetics for 36 h after infecting CD4(+) T cells obtained from the blood of twelve healthy donors. The epigenetic targets aurora kinase B (AURKB), aurora kinase C (AURKC) and DNA methyltransferase 3B (DNMT3B), and the global DNA methylation profile are regulated during HIV-1 replication in CD4(+) T cells, and this regulation can be influenced by the activation state of the cell at the time of infection. Approaches that affect the expression of these epigenetic targets could help current strategies to suppress HIV-1 replication. (AU)

• FAPESP's process: 12/12830-5 - Study of modulation of epigenetic factors involved in the early stages of infection by HIV-1 in resting TCD4+ cells
• Grantee: Luiz Mário Ramos Janini
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/09678-0 - Study of the modulation of epigenetic factors involved in the early stages of HIV-1 infection in resting TCD4+ cells
• Grantee: Jorge Meneses Nunes
• Support Opportunities: Scholarships in Brazil - Doctorate