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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Rare RELN variants affect Reelin-DAB1 signal transduction in autism spectrum disorder

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Sanchez-Sanchez, Sandra M. [1, 2] ; Magdalon, Juliana [2] ; Griesi-Oliveira, Karina [2] ; Yamamoto, Guilherme L. [1] ; Santacruz-Perez, Carolina [3] ; Fogo, Mariana [1, 2] ; Passos-Bueno, Maria Rita [1] ; Sertie, Andrea L. [2]
Total Authors: 8
[1] Univ Sao Paulo, Dept Genet & Evolutionary Biol, Sao Paulo - Brazil
[2] Hosp Israelita Albert Einstein, Ctr Expt Res, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Human mutation; v. 39, n. 10, p. 1372-1383, OCT 2018.
Web of Science Citations: 3

The Reelin-DAB1 signaling pathway plays a crucial role in regulating neuronal migration and synapse function. Although many rare heterozygous variants in the Reelin gene (RELN) have been identified in patients with autism spectrum disorder (ASD), most variants are still of unknown clinical significance. Also, genetic data suggest that heterozygous variants in RELN alone appear to be insufficient to cause ASD. Here, we describe the identification and functional characterization of rare compound heterozygous missense variants in RELN in a patient with ASD in whom we have previously reported hyperfunctional mTORC1 signaling of yet unknown etiology. Using iPSC-derived neural progenitor cells (NPCs) from this patient, we provide experimental evidence that the identified variants are deleterious and lead to diminished Reelin secretion and impaired Reelin-DAB1 signal transduction. Also, our results suggest that mTORC1 pathway overactivation may function as a second hit event contributing to downregulation of the Reelin-DAB1 cascade in patient-derived NPCs, and that inhibition of mTORC1 by rapamycin attenuates Reelin-DAB1 signaling impairment. Taken together, our findings point to an abnormal interplay between Reelin-DAB1 and mTORC1 networks in nonsyndromic ASD. (AU)

FAPESP's process: 14/25646-3 - Investigation of the cellular and molecular effects of RELN mutations in Autism Spectrum Disorders.
Grantee:Andréa Laurato Sertié
Support Opportunities: Regular Research Grants
FAPESP's process: 15/50138-4 - Elucidating the role of the innate immune complement pathway in brain development and in Autism Spectrum Disorders
Grantee:Andréa Laurato Sertié
Support Opportunities: Regular Research Grants