| Full text | |
| Author(s): |
de Braganca, Ana Carolina
[1]
;
Canale, Daniele
[2]
;
Goncalves, Janaina Garcia
[2]
;
Massola Shimizu, Maria Heloisa
[2]
;
Seguro, Antonio Carlos
[1]
;
Volpini, Rildo Aparecido
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, LIM12, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med, LIM12, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | FRONTIERS IN MEDICINE; v. 5, OCT 10 2018. |
| Web of Science Citations: | 3 |
| Abstract | |
The pathogenesis of chronic kidney disease (CKD) involves a very complex interaction between hemodynamic and inflammatory processes, leading to glomerular/vascular sclerosis, and fibrosis formation with subsequent evolution to end-stage of renal disease. Despite efforts to minimize the progression of CKD, its incidence and prevalence continue to increase. Besides cardiovascular diseases and infections, several studies demonstrate that vitamin D status could be considered as a non-traditional risk factor for the progression of CKD. Therefore, we investigated the effects of vitamin D deficiency (VDD) in the course of moderate CKD in 5/6 nephrectomized rats (Nx). Adult male Wistar rats underwent Sham surgery or Nx and were subdivided into the following four groups: Sham, receiving standard diet (Sham); Sham VDD, receiving vitamin D-free diet (VDD); Nx, receiving standard diet (Nx); and VDD+Nx, receiving vitamin D-free diet (VDD+Nx). Sham or Nx surgeries were performed 30 days after standard or vitamin D-free diets administration. After validation of vitamin D depletion, we considered only Nx and VDD+Nx groups for the following studies. Sixty days after surgeries, VDD+Nx rats exhibited hypertension, a greater decline in renal function and plasma FGF-23 levels, renal hypertrophy, as well as higher plasma levels of PTH and aldosterone. In addition, those animals presented more significant chronic tubulointerstitial changes (cortical interstitial expansion/inflammation/fibrosis), higher expression of collagen IV, fibronectin and alpha-smooth muscle actin, and lower expressions of JG12 and M2 macrophages. Also, VDD+Nx rats had greater infiltration of inflammatory cells (M1 macrophages and T-cells). Such changes were accompanied by higher expression of TGF-beta 1 and angiotensinogen and decreased expression of VDR and Klotho protein. Our observations indicate that vitamin D deficiency impairs the renal function and worsens the renovascular and morphological changes, aggravating the features of moderate CKD in 5/6 nephrectomized rats. (AU) | |
| FAPESP's process: | 10/52294-0 - Assessing the role of vitamin D in the development of postischemic acute kidney injury |
| Grantee: | Rildo Aparecido Volpini |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/05513-1 - Evaluation of the events involved in the renal changes associated with experimental models of ischemic acute kidney injury, 5/6 nephrectomy and nephrotoxicity by Amphotericin B: influence of vitamin D deficiency |
| Grantee: | Rildo Aparecido Volpini |
| Support Opportunities: | Regular Research Grants |