Solid lipid nanoparticles optimized by 2(2) factorial design for skin administration: Cytotoxicity in NIH3T3 fibroblasts

Author(s)

Rigon, Roberta Balansin ^[1] ; Goncalez, Maira Lima ^[1] ; Severino, Patricia ^[2] ; Alves, Danilo Antonini ^[3] ; Santana, Maria H. A. ^[4] ; Souto, Eliana B. ^{[5, 6]} ; Chorilli, Marius ^[1]

Affiliation

^[1] UNESP Sao Paulo State Univ, Fac Pharmaceut Sci, Dept Farmacos & Medicamentos, Campus Araraquara, BR-14800850 Araraquara, SP - Brazil

^[2] Univ Tiradentes, Ctr Biol Sci & Hlth, BR-49010390 Aracaju, Sergipe - Brazil

^[3] Campinas Univ UNICAMP, Inst Biol, Lab Biotechnol, BR-13083862 Campinas, SP - Brazil

^[4] Campinas Univ UNICAMP, Fac Chem Engn, BR-13083970 Campinas, SP - Brazil

^[5] Univ Coimbra FFUC, Fac Pharm, Dept Pharmaceut Technol, Polo Ciencias Saude Azinhaga Santa Comba, P-3000548 Coimbra - Portugal

^[6] Univ Coimbra, Fac Pharm, Grp Pharmaceut Technol, REQUIMTE LAQV, Coimbra - Portugal

• Document type: Journal article
• Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 171, p. 501-505, NOV 1 2018.
• Web of Science Citations: 7

Abstract

The present study focuses on the characterization of the cytotoxic profile on NIH3T3 mouse embryonic fibroblasts of solid lipid nanoparticles (SLN) optimized by a 2(2) full factorial design for skin administration. To build up the surface response charts, a design of experiments (DoE) based on 2 independent variables was used to obtain an optimized formulation. The effect of the composition of lipid and water phases on the mean particle size (z-AVE), polydispersity index (PdI) and zeta potential (ZP) was studied. The developed formulations were composed of 5.0% of lipid phase (stearic acid (SA), behenic alcohol (BA) or a blend of SA:BA (1:1)) and 4.7% of surfactants (soybean phosphatidylcholine and poloxamer 407). In vitro cytotoxicity using NIH3T3 fibroblasts was performed by MTT reduction assay. This factorial design study has proven to be a useful tool in optimizing SLN (z-AVE similar to 200 nm), which were shown to be non-cytotoxic. The present results highlight the benefit of applying statistical designs in the preparation and optimization of SLN formulations. (AU)

• FAPESP's process: 13/21500-1 - Solid lipid nanoparticles for incorporation of trans-resveratrol in the topical treatment of melanoma: in vitro and in vivo evaluation and the elucidation of the mechanism of uptake cellular
• Grantee: Roberta Balansin Rigon
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/19568-4 - Evaluation of the potential of nanostructured lipid systems for cutaneous administration of trans-resveratrol
• Grantee: Marlus Chorilli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/16888-5 - Development and characterization of solid lipid nanoparticles for dermal administration of trans-resveratrol
• Grantee: Roberta Balansin Rigon
• Support Opportunities: Scholarships in Brazil - Master