| Full text | |
| Author(s): |
Martins Oliveira-Brito, Patricia Kellen
[1]
;
Roque-Barreira, Maria Cristina
[1]
;
da Silva, Thiago Aparecido
[1]
Total Authors: 3
|
| Affiliation: | [1] Univ Sao Paulo, Dept Biol Celular & Mol & Bioagentes Patogen, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Molecules; v. 23, n. 9 SEP 2018. |
| Web of Science Citations: | 0 |
| Abstract | |
ArtinM, a D-mannose-binding lectin from Artocarpus heterophyllus, activates antigen-presenting cells by recognizing Toll-like receptor (TLR) 2 and cluster of differentiation (CD) 14 N-glycans, induces cytokine production, and promotes type 1 T helper (Th1) immunity, a process that plays an assisting role in the combat against fungal infections. We recently demonstrated that ArtinM stimulates CD4(+) T cells to produce interleukin (IL)-17 through direct interaction with CD3. Here, we further investigated the effects of ArtinM on the production of IL-17 by B cell activation. We showed that ArtinM activates murine B cells, increasing IL-17 and IL-12p40 production. The direct effect of ArtinM was sufficient to induce IL-17 production in B cells, and we did not find differences in the levels of IL-17 between the B cells purified from the wild-type (WT) and knockout (KO) mice for TLR2 or CD14 in the presence of ArtinM. Thus, the effects of ArtinM on splenic B cells through carbohydrate recognition may contribute to Th17 immunity; however, the mechanism involved is not associated with the interaction of ArtinM with TLR2 and CD14. The current work represents a pioneering effort in the understanding of the induction of IL-17 by lectins in B cells. (AU) | |
| FAPESP's process: | 16/23112-7 - The immunomodulatory activity of ArtinM on the course of Cryptococcus gattii infection |
| Grantee: | Patrícia Kellen Martins Oliveira Brito |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 16/04877-2 - Design of new therapeutic strategies, based on the carbohydrate recognition, against cryptococcosis |
| Grantee: | Thiago Aparecido da Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/10446-4 - Mechanisms of immunomodulation by ArtinM: basis for the development of new anti-fungal therapy |
| Grantee: | Maria Cristina Roque Antunes Barreira |
| Support Opportunities: | Regular Research Grants |