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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Posttranscriptional actions of triiodothyronine on Tshb expression in T alpha T1 cells: New insights into molecular mechanisms of negative feedback

Full text
Author(s):
Bargi-Souza, Paula [1] ; Goulart-Silva, Francemilson [1] ; Nunes, Maria Tereza [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Molecular and Cellular Endocrinology; v. 478, p. 45-52, DEC 15 2018.
Web of Science Citations: 1
Abstract

Rapid actions of triiodothyronine (T3) on thyrotropin (TSH) synthesis and secretion have been described in hypothyroid male rats. However, the molecular mechanisms remain unknown. T alpha T1 cells, a thyrotroph cell line, was used herein to characterize the possible non-genomic actions of T3 on the expression of alpha (Cga) and Tshb genes, and the posttranscriptional processing and translation of both transcripts. The involvement of alpha V beta 3 integrin was also assessed. T3 quickly reduced Tshb mRNA content, poly(A) tail length and its association with ribosomes. The effect of T3 on Tshb gene expression was detected even in the presence of a transcription inhibitor. The decrease in Tshb mRNA content and polyadenylation depend on T3 interaction with alpha V beta 3 integrin, while T3 reduced Cga mRNA content by transcriptional action. The translational rate of both transcripts was reduced by a mechanism, which does not depend on T3-alpha V beta 3 integrin interaction. Results indicate that, in parallel with the inhibitory transcriptional action in Cga and Tshb gene expression, T3 rapidly triggers additional posttranscriptional mechanisms, reducing the TSH synthesis. These non-genomic actions partially depend on T3-alpha V beta 3 integrin interaction at the plasma membrane of thyrotrophs and add new insights to the molecular mechanisms involved in T3 negative feedback loop. (AU)

FAPESP's process: 13/05629-4 - Genomic vs nongenomic actions of thyroid hormones: changes of paradigms, physiological implications and therapeutical perspectives
Grantee:Maria Tereza Nunes
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 09/17813-9 - Rapid actions of Triiodothyronine (T3) on TSH expression and secretion: new mechanisms involved in the negative feedback.
Grantee:Paula Bargi de Souza
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/25888-7 - Characterization of the molecular basis involved on the expression of circadian clock genes in the pituitary at different thyroid states and the consequences for the synthesis/secretion of anterior pituitary hormones
Grantee:Paula Bargi de Souza
Support Opportunities: Scholarships in Brazil - Post-Doctoral