Increased Soluble Cytoplasmic Bcl-2 Protein Serum Levels and Expression and Decreased Fas Expression in Lymphocytes and Monocytes in Juvenile Dermatomyositis

Author(s)

Liphaus, Bernadete L. ^[1] ; Sallum, Adriana E. M. ^[2] ; Aikawa, Nadia E. ^[2] ; Kiss, Maria Helena B. ^[3] ; Carrasco, Solange ^[4] ; Palmeira, Patricia ^[4] ; Lima, Laila ^[1] ; Silva, Clovis A. ^{[4, 2]} ; Goldenstein-Schainberg, Claudia ^[4] ; Carneiro-Sampaio, Magda ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Med, Childrens Inst, Lab Med Invest, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Childrens Inst, Pediat Rheumatol Unit, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF RHEUMATOLOGY; v. 45, n. 11, p. 1577-1580, NOV 1 2018.
• Web of Science Citations: 1

Abstract

Objective. To evaluate soluble Fas antigen (sFas), sFas ligand (sFasL), soluble tumor necrosis factor-related apoptosis-inducing ligand, and soluble cytoplasmic Bcl-2 protein (sBcl-2) serum levels, Fas and Bcl-2 expressions in T and B lymphocytes and monocytes and relations with erythrocyte sedimentation rate, C-reactive protein (CRP), Childhood Myositis Assessment Scale, and manual muscle testing in juvenile dermatomyositis (JDM). Methods. Serum levels were determined by ELISA and peripheral cell expressions by flow cytometry for patients with JDM or juvenile idiopathic arthritis (JIA), and healthy controls. Results. Patients with JDM had increased sBcl-2, which correlated with CRP. Expression of Bcl-2 was increased and expression of Fas was decreased in CD3+, CD4+, and CD8+ T lymphocytes compared with JIA and/or healthy controls. Conclusion. Patients with JDM presented a unique apoptosis-related proteins profile, which may contribute to disease development. (AU)

• FAPESP's process: 12/22997-4 - Evaluation of apoptosis related proteins in lymphocytes, monocytes and NK cells from patients with juvenile systemic lupus erythematosus
• Grantee: Bernadete de Lourdes Liphaus
• Support type: Regular Research Grants
• FAPESP's process: 08/58238-4 - Autoimmunity in children: investigation of the molecular and cellular bases of early onset of autoimmunity
• Grantee: Magda Maria Sales Carneiro-Sampaio
• Support type: Research Projects - Thematic Grants