2-Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L.

Author(s)

Alves, Ivanilda dos Santos ^[1] ; Barbosa Carmazini, Valeria Cristina ^[1] ; dos Santos, Cosme Damiao ^[2] ; Silva de Almeida, Julieta Andrea ^[1]

Affiliation

^[1] Inst Agron Campinas, Ctr Cafe Alcides Carvalho, BR-13020902 Campinas, SP - Brazil

^[2] Escola Tecn Conselheiro Antonio Prado ETECAP, Ctr Paula Souza, Campinas, SP - Brazil

• Document type: Journal article
• Source: Ciência Rural; v. 48, n. 11 2018.
• Web of Science Citations: 0

Abstract

This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL(-1)) and 2-iP (0; 2.5; 5: 7.5 and 10 mu M). The 2-iP doses of 7.5 and 10 mu M produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However. the greatest production of somatic embryos occurred on the explants treated with 10 mu M of 2-iP. followed by 7.5 mu M. whereas their production was absent or reduced with 0 and 5 mu M. respectively. (AU)

• FAPESP's process: 11/10090-1 - Osmotic stress in the control of direct somatic embryogenesis em genotypes of Coffea arabica
• Grantee: Julieta Andrea Silva de Almeida
• Support Opportunities: Regular Research Grants