| Full text | |
| Author(s): |
Aranda Salomao, Priscila Maria
[1]
;
de Oliveira, Flavia Amadeu
[1]
;
Soares dos Santos, Daiana Moreli
[1]
;
Domezi, Joao Paulo
[1]
;
Dionisio, Thiago Jose
[1]
;
de Oliveira, Rodrigo Cardoso
[1]
;
Magalhaes, Ana Carolina
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Al Dr Octavio Pinheiro Brisolla 9-75, Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | ARCHIVES OF ORAL BIOLOGY; v. 97, p. 245-252, JAN 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
Objectives: This study evaluated the level and mechanism of apoptosis in human gingival fibroblasts (HGF) and murine fibroblasts (NIH/3T3) treated with a titanium tetrafluoride (TiF4) varnish compared those treated with a sodium fluoride (NaF) varnish. Methods: Cells were treated with a TiF4, NaF (both 2.45%F) or placebo varnish for 6 h and were then examined using the TUNEL method. The activities of caspase-3, -8 and -9 were assessed. cDNA for Bax, Bad, Bcl-2 and Fas-L was amplified by quantitative PCR. Bax, Bcl-2 and Fas-L were further detected by western blot analysis. Results: Both fluorides similarly increased the percentage of apoptosis, while they failed to activate caspases. The Bax/Bcl-2 gene expression ratio was not altered by either fluoride treatment regardless of the type of cell. NaF varnish increased the amplification of the Fas-L gene in NIH/3T3 and HGF cells, while treatment with the TiF4 varnish resulted in a lower Bad/Bcl-2 expression ratio compared to that of the control for NIH/3T3 cells, but not for HGF cells. No effect of the fluorides was detected in the protein analysis. Conclusions: NaF and TiF4, at the studied conditions, similarly induce a low level of apoptosis, with consequent modest activation of the Bcl-2 and Fas-L-dependent signalling pathways. Generally, HGF cells are more susceptible to the fluoride effect than NIH/3T3 cells. (AU) | |
| FAPESP's process: | 13/13484-6 - Effect of titanium tetrafluoride varnish on fibroblasts (NIH3T3): viability assay, morphological analysis and cell signals for apoptotic pathways |
| Grantee: | Priscila Maria Aranda Salomão |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/20372-0 - Effect of titanium tetrafluoride varnish on fibroblasts (NIH3T3): viability assay, morphological analysis and cell signals for apoptotic pathways |
| Grantee: | Ana Carolina Magalhães |
| Support Opportunities: | Regular Research Grants |