Transferability of nuclear microsatellite markers to the atmospheric bromeliads Tillandsia recurvata and T-aeranthos (Bromeliaceae)

Author(s)

Neves Chaves, Cleber Juliano ^[1] ; Aoki-Goncalves, Felipe ^[1] ; Santos Leal, Barbara Simoes ^[1] ; Rossatto, Davi Rodrigo ^{[1, 2]} ; Palma-Silva, Clarisse ^{[3, 1]}

Affiliation

^[1] Univ Estadual Paulista Unesp, Inst Biociencias, Programa Posgrad Ecol & Biodiversidad, BR-13506900 Rio Claro - Brazil

^[2] Univ Estadual Paulista Unesp, Dept Biol, BR-14884900 Jaboticabal - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13083862 Campinas, SP - Brazil

• Document type: Journal article
• Source: BRAZILIAN JOURNAL OF BOTANY; v. 41, n. 4, p. 931-935, DEC 2018.
• Web of Science Citations: 2

Abstract

The genus Tillandsia L. is the most diversified genus of Bromeliaceae and represents one of the most specialized cases of vascular epiphytism: the atmospheric bromeliads.' Such great diversity and ecological specialization make it an interesting model for evolutionary and population genetics studies. Here we report the cross-transferability of SSR markers isolated from other bromeliad species to Tillandsia recurvata (L.) L. and T. aeranthos (Loisel.) Desf., epiphytes with great abundance in both natural and anthropogenic-modified environments, but with contrasting patterns of geographic distribution and mating systems. We tested a total of 27 microsatellite markers and successfully amplified seven polymorphic markers in T. recurvata and T. aeranthos. We then described cross-amplified markers in two populations per species, sampled in both anthropogenic-transformed and natural environments. T. recurvata presented lower allelic richness and heterozygosities, and greater inbreeding coefficient values. Such differences clearly reflect their contrasting mating systems (self-fertilizing in T. recurvata versus self-incompatible in T. aeranthus). The set of cross-amplified microsatellite markers described here will be a helpful tool to address a range of evolutionary and ecological questions. (AU)

• FAPESP's process: 16/04396-4 - COMMUNITIES DOMINATED BY ATMOSPHERIC BROMELIADS AS A MODEL FOR METACOMMUNITIES STUDIES
• Grantee: Cleber Juliano Neves Chaves
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/15588-6 - Phylogeography, population genomics and adaptive variation in Pitcairnia lanuginosa (Bromeliaceae) species complex
• Grantee: Clarisse Palma da Silva
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/08087-0 - Phylogeography, adaptive variation and reproductive biology of Pitcairnia lanuginosa complex (Bromeliaceae)
• Grantee: Bárbara Simões Santos Leal
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/03777-4 - Patterns of genetic diversity and phylogeography of Tillandsia aeranthos (Lois) L. B. Smith (Bromeliaceae)
• Grantee: Felipe Aoki Gonçalves
• Support Opportunities: Scholarships in Brazil - Master