Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii

Author(s)

de Oliveira Cardoso, Marystela Favero ^{[1, 2]} ; Moreli, Jusciele Brogin ^[1] ; Gomes, Angelica Oliveira ^[3] ; Zanon, Caroline de Freitas ^[4] ; Silva, Ana Elizabete ^[4] ; Paulesu, Luana Ricci ^[5] ; Ietta, Francesca ^[5] ; Mineo, Jose Roberto ^[6] ; Ferro, Eloisa Amalia ^[7] ; Oliani, Sonia Maria ^{[1, 4]}

Affiliation

^[1] Fed Univ Sao Paulo UNIFESP, Postgrad Struct & Funct Biol, Sao Paulo, SP - Brazil

^[2] Faceres Sch Med, Sao Jose Do Rio Preto, SP - Brazil

^[3] Fed Univ Triangulo Mineiro UFTM, Inst Biol & Nat Sci, Dept Struct Biol, Uberaba, MG - Brazil

^[4] Sao Paulo State Univ UNESP, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil

^[5] Univ Siena, Dept Life Sci, Siena - Italy

^[6] Fed Univ Uberlandia UFU, Dept Immunol Microbiol & Parasitol, Uberlandia, MG - Brazil

^[7] Fed Univ Uberlandia UFU, Dept Histol & Embryol, Uberlandia, MG - Brazil

• Document type: Journal article
• Source: Microbial Pathogenesis; v. 123, p. 153-161, OCT 2018.
• Web of Science Citations: 1

Abstract

This study was conducted to investigate annexin A1 (ANXA1) functions in human placental explants infected with Toxoplasma gondii (T. gondii). We examined the first and third trimester placental explants infected with T. gondii (n = 7 placentas/group) to identify the number and location of parasites, ANXA1 protein, potential involvement of formyl peptide receptors (FPR1 and FPR2), and COX-2 expressions by immunohistochemistry. Treatments with Ace-26 mimetic peptide of ANXA1 were performed to verify the parasitism rate (beta-galactosidase assay), prostaglandin E-2 levels (ELISA assay), and ANXA1, FPR1 and COX-2 expression in third trimester placentas. Placental explants of third trimester expressed less ANXA1 and were more permissive to T. gondii infection than first trimester placentas that expressed more ANXA1. AC(2-26) treatment increases endogenous ANXA1 and decreases parasitism rate, COX-2, and prostaglandin E-2 levels. Altogether, these data provide further insight into the anti-parasitic and anti-inflammatory effects of ANXA1 in placentas infected with T. gondii. (AU)

• FAPESP's process: 16/02012-4 - Evaluation of the immunomodulatory activity of annexin A1 protein in the regulation of inflammatory disorders of the gastrointestinal system: studies in vivo and in vitro experimental models
• Grantee: Sonia Maria Oliani
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/08273-6 - STUDY OF ANTI-INFLAMMATORY PROTEIN ANNEXIN A1 IN EXPLANT CULTURES OF HUMAN PLACENTAL VILOS INFECTED BY TOXOPLASMA GONDII
• Grantee: Marystela Fávero de Oliveira Cardoso
• Support Opportunities: Scholarships in Brazil - Master