| Full text | |
| Author(s): |
Siden-Kiamos, Inga
[1]
;
Pace, Tomasino
[2]
;
Klonizakis, Antonios
[1, 3]
;
Nardini, Marco
[4]
;
Garcia, Celia R. S.
[5]
;
Curra, Chiara
[1]
Total Authors: 6
|
| Affiliation: | [1] Fdn Res & Technol Hellas, Inst Mol Biol & Biotechnol, N Plastira 100, GR-70013 Iraklion - Greece
[2] Ist Super Sanita, Dipartimento Malattie Infett Parassitarie & Immun, I-00161 Rome - Italy
[3] Univ Crete, Biol Dept, Voutes Univ Campus, GR-70013 Iraklion - Greece
[4] Univ Milan, Dept BioSci, Via Celoria 26, I-20133 Milan - Italy
[5] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Av Prof Lineu Prestes 580, BR-05508090 Sao Paulo, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | International Journal for Parasitology; v. 48, n. 14, p. 1127-1136, DEC 2018. |
| Web of Science Citations: | 2 |
| Abstract | |
Sporozoites are the infective form of malaria parasites which are transmitted from the mosquito salivary glands to a new host in a mosquito blood meal. The sporozoites develop inside the sporogonic oocyst and it is crucial for the continuation of the life cycle that the oocyst ruptures to release sporozoites. We recently described two Plasmodium Oocyst Rupture Proteins (ORP1 and ORP2), localized at the oocyst capsule, that are each essential for rupture of the oocysts. Both ORPs contain a histone fold domain implicated in the mechanism of oocyst rupture, possibly through the formation of a heterodimer between the two histone fold domains. To gain an understanding of the function of the different regions of the ORP2 protein, we generated deletion mutants. We monitored oocyst formation and rupture as well as sporozoites in the salivary gland. Our results show that different regions of ORP2 play independent roles in sporozoite egress. Deleting the N-terminal histone fold domain of ORP2 blocked sporozoite egress from the oocyst. Progressive deletions from the C-terminal resulted in no or significantly impaired sporozoite egress. (C) 2018 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 16/03952-0 - Role of two histone-fold domain proteins in Plasmodium parasites |
| Grantee: | Chiara Currà |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |