In Vivo Study of the Action of a Topical Anti-Inflammatory Drug In Rat Teeth Submitted To Dental Bleaching

Author(s)

Francine Benetti ^[1] ; André Luiz Fraga Briso ^[2] ; Luciana Louzada Ferreira ^[3] ; Marina Carminatti ^[4] ; Larissa Álamo ^[5] ; Edilson Ervolino ^[6] ; Eloi Dezan-Júnior ^[7] ; Luciano Tavares Angelo Cintra ^[8]

Affiliation

^[1] UNESP - Universidade Estadual Paulista. Endodontics, Araçatuba Dental School - Brasil

^[2] UNESP - Universidade Estadual Paulista. Restorative Dentistry, Araçatuba Dental School - Brasil

^[3] UNESP - Universidade Estadual Paulista. Endodontics, Araçatuba Dental School - Brasil

^[4] UNESP - Universidade Estadual Paulista. Endodontics, Araçatuba Dental School - Brasil

^[5] UNESP - Universidade Estadual Paulista. Endodontics, Araçatuba Dental School - Brasil

^[6] UNESP - Universidade Estadual Paulista. Basic Science, Araçatuba Dental School - Brasil

^[7] UNESP - Universidade Estadual Paulista. Endodontics, Araçatuba Dental School - Brasil

^[8] UNESP - Universidade Estadual Paulista. Endodontics, Araçatuba Dental School - Brasil

• Document type: Journal article
• Source: Brazilian Dental Journal; v. 29, n. 6, p. 555-561, 2018-12-00.

Abstract

Abstract Bleaching gel containing hydrogen peroxide (H2O2) cause damages in pulp tissue. This study investigated the action of a topical anti-inflammatory, the Otosporin®, in rats’ bleached teeth with the null hypothesis of which the Otosporin® is no able to minimize the pulp inflammation that bleaching gel generates. The rat’s molars were divided into groups: BLE: bleached (35% H2O2 concentration /single application of 30 min); BLE-O: bleached followed by Otosporin® (10 min); and control: placebo gel. In the second day after dental bleaching, the rats were killed, and the jaws were processed for hematoxylin-eosin and immunohistochemistry analysis for tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-17. The data collected were subjected to Kruskal-Wallis and Dunn statistical tests with at a 5% level of significance (p<0.05). The BLE group had moderate to strong inflammation in the occlusal third of the coronary pulp, with necrotic areas; and BLE-O, mild inflammation (p<0.05). There was a significant difference in the occlusal and middle thirds of the coronary pulp between the BLE with BLE-O and control groups (p<0.05). There was no difference in the cervical third (p>0.05). The BLE group had a high immunoexpression of TNF-α than BLE-O and control groups (p<0.05), with moderate and mild immunoexpression, respectively. Regarding IL-6 and IL-17, the BLE group had higher immunoexpression than control (p<0.05); the BLE-O was similar to the control (p>0.05). The topical anti-inflammatory Otosporin® can reduce pulp inflammation after dental bleaching in the rat teeth. (AU)

• FAPESP's process: 15/10825-2 - Influence of Hydrogen Peroxide on cell differentiation and mineralization of the pulp tissue after bleaching procedure. Histological and immunohistochemical study.
• Grantee: Francine Benetti
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/09736-2 - In vivo study of the anti-inflammatory active of Otosporin in rat's teeth submitted to dental bleaching: histological and immunohistochemistry analysis
• Grantee: Marina Carminatti
• Support Opportunities: Scholarships in Brazil - Scientific Initiation