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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In Vivo Study of the Action of a Topical Anti-Inflammatory Drug In Rat Teeth Submitted To Dental Bleaching

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Author(s):
Francine Benetti [1] ; André Luiz Fraga Briso [2] ; Luciana Louzada Ferreira [3] ; Marina Carminatti [4] ; Larissa Álamo [5] ; Edilson Ervolino [6] ; Eloi Dezan-Júnior [7] ; Luciano Tavares Angelo Cintra [8]
Total Authors: 8
Affiliation:
[1] UNESP - Universidade Estadual Paulista. Endodontics, Araçatuba Dental School - Brasil
[2] UNESP - Universidade Estadual Paulista. Restorative Dentistry, Araçatuba Dental School - Brasil
[3] UNESP - Universidade Estadual Paulista. Endodontics, Araçatuba Dental School - Brasil
[4] UNESP - Universidade Estadual Paulista. Endodontics, Araçatuba Dental School - Brasil
[5] UNESP - Universidade Estadual Paulista. Endodontics, Araçatuba Dental School - Brasil
[6] UNESP - Universidade Estadual Paulista. Basic Science, Araçatuba Dental School - Brasil
[7] UNESP - Universidade Estadual Paulista. Endodontics, Araçatuba Dental School - Brasil
[8] UNESP - Universidade Estadual Paulista. Endodontics, Araçatuba Dental School - Brasil
Total Affiliations: 8
Document type: Journal article
Source: Brazilian Dental Journal; v. 29, n. 6, p. 555-561, 2018-12-00.
Abstract

Abstract Bleaching gel containing hydrogen peroxide (H2O2) cause damages in pulp tissue. This study investigated the action of a topical anti-inflammatory, the Otosporin®, in rats’ bleached teeth with the null hypothesis of which the Otosporin® is no able to minimize the pulp inflammation that bleaching gel generates. The rat’s molars were divided into groups: BLE: bleached (35% H2O2 concentration /single application of 30 min); BLE-O: bleached followed by Otosporin® (10 min); and control: placebo gel. In the second day after dental bleaching, the rats were killed, and the jaws were processed for hematoxylin-eosin and immunohistochemistry analysis for tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-17. The data collected were subjected to Kruskal-Wallis and Dunn statistical tests with at a 5% level of significance (p<0.05). The BLE group had moderate to strong inflammation in the occlusal third of the coronary pulp, with necrotic areas; and BLE-O, mild inflammation (p<0.05). There was a significant difference in the occlusal and middle thirds of the coronary pulp between the BLE with BLE-O and control groups (p<0.05). There was no difference in the cervical third (p>0.05). The BLE group had a high immunoexpression of TNF-α than BLE-O and control groups (p<0.05), with moderate and mild immunoexpression, respectively. Regarding IL-6 and IL-17, the BLE group had higher immunoexpression than control (p<0.05); the BLE-O was similar to the control (p>0.05). The topical anti-inflammatory Otosporin® can reduce pulp inflammation after dental bleaching in the rat teeth. (AU)

FAPESP's process: 15/10825-2 - Influence of Hydrogen Peroxide on cell differentiation and mineralization of the pulp tissue after bleaching procedure. Histological and immunohistochemical study.
Grantee:Francine Benetti
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/09736-2 - In vivo study of the anti-inflammatory active of Otosporin in rat's teeth submitted to dental bleaching: histological and immunohistochemistry analysis
Grantee:Marina Carminatti
Support Opportunities: Scholarships in Brazil - Scientific Initiation