In Vitro Antimicrobial Effect of Bioadhesive Oral Membrane with Chlorhexidine Gel

Author(s)

Annelyze Podolan Kloster ^[1] ; Natalino Lourenço Neto ^[2] ; Silgia Aparecida da Costa ^[3] ; Thais Marchini Oliveira ^[4] ; Rodrigo Cardoso de Oliveira ^[5] ; Maria Aparecida Andrade Moreira Machado ^[6]

Affiliation

^[1] Universidade de São Paulo. Bauru Dental School. Department of Pediatric Dentistry, Orthodontics and Community Dentistry - Brasil

^[2] Universidade de São Paulo. Bauru Dental School. Department of Pediatric Dentistry, Orthodontics and Community Dentistry - Brasil

^[3] Universidade de São Paulo. School of Arts, Sciences and Humanities. Course on Textiles and Fashion - Brasil

^[4] Universidade de São Paulo. Bauru Dental School. Department of Pediatric Dentistry, Orthodontics and Community Dentistry - Brasil

^[5] Universidade de São Paulo. Bauru Dental School. Department of Biological Sciences, Discipline of Biochemistry - Brasil

^[6] Universidade de São Paulo. Bauru Dental School. Department of Pediatric Dentistry, Orthodontics and Community Dentistry - Brasil

• Document type: Journal article
• Source: Brazilian Dental Journal; v. 29, n. 4, p. 354-358, 2018-08-00.

Abstract

Abstract This study aimed to evaluate in vitro the antimicrobial effect of a bioadhesive chitosan-based oral membrane with chlorhexidine for local treatment of infections in the oral tissues. Five oral membranes of different compositions were tested: 5% chitosan (G1); 5% chitosan ± 0.2% chlorhexidine (G2), 5% chitosan ± 0.6% chlorhexidine (G3), 5% chitosan ± 1.0% chlorhexidine (G4), and 5% chitosan ± 2.0% chlorhexidine (G5). Also, five gel types were tested according to the following compositions: 5% chitosan gel (G6), 0.2% chlorhexidine gel (G7), 2.0% chlorhexidine gel (G8), 5% chitosan gel ± 0.2% chlorhexidine gel (G9), and 5% chitosan gel ± 2.0% chlorhexidine gel (G10). The antimicrobial action of the samples was tested against Candida albicans and Streptococcus mutans through antibiogram by measuring the inhibition halos. Data were statistically analyzed by Kruskal-Wallis and one-way ANOVA followed by Tukey test (p<0.05). The 2.0% chlorhexidine membrane (G5) and the disks containing 2.0% chlorhexidine gel (G8) showed the greatest inhibition halos for both microorganisms, with statistically significant difference when compared to others tested groups (p=0.008) only for Candida albicans inhibitions results. All the other formulations of membranes and gels showed inhibition halos, but without statistically significant difference. The bioadhesive chitosan-based oral membrane with 2% chlorhexidine and 2% chlorhexidine gel were the most effective in inhibiting the tested microorganisms. (AU)

• FAPESP's process: 13/07836-7 - Analysis and development of a dentin/pulpar biostimulating bandage (BBio)
• Grantee: Natalino Lourenço Neto
• Support Opportunities: Scholarships in Brazil - Post-Doctoral