2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L.

Author(s)

Ivanilda dos Santos Alves ^[1] ; Valéria Cristina Barbosa Carmazini ^[2] ; Cosme Damião dos Santos ^[3] ; Julieta Andrea Silva de Almeida ^[4]

Affiliation

^[1] Instituto Agronômico de Campinas (IAC). Centro de Café ‘Alcides Carvalho’ - Brasil

^[2] Instituto Agronômico de Campinas (IAC). Centro de Café ‘Alcides Carvalho’ - Brasil

^[3] Centro Paula Souza. Escola Técnica Conselheiro Antônio Prado (ETECAP) - Brasil

^[4] Instituto Agronômico de Campinas (IAC). Centro de Café ‘Alcides Carvalho’ - Brasil

• Document type: Journal article
• Source: Ciência Rural; v. 48, n. 11 2018-11-14.

Abstract

ABSTRACT: This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and 2-iP (0; 2.5; 5; 7.5 and 10µM). The 2-iP doses of 7.5 and 10µM produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However, the greatest production of somatic embryos occurred on the explants treated with 10µM of 2-iP, followed by 7.5µM, whereas their production was absent or reduced with 0 and 5µM, respectively. (AU)

• FAPESP's process: 11/10090-1 - Osmotic stress in the control of direct somatic embryogenesis em genotypes of Coffea arabica
• Grantee: Julieta Andrea Silva de Almeida
• Support Opportunities: Regular Research Grants