Picrolemma sprucei quassinosides inhibits breast and prostate cell growth and impairs behavioral phenotype in mice

Author(s)

Nogaia de Sousa, Sinaria Rejany ^[1] ; Collantes Diaz, Ingrit Elida ^[2] ; Younes, Riad Naim ^[3] ; Frana, Sergio Alexandre ^{[4, 5]} ; Bernardi, Maria Martha ^[1] ; Suffredini, Ivana Barbosa ^{[1, 3]}

Affiliation

^[1] Univ Paulista, Grad Program Environm & Expt Pathol, Sao Paulo - Brazil

^[2] Univ Nacl Ingn, Fac Ingn Quim & Text, Dept Ingn Quim, Lima - Peru

^[3] Univ Paulista, Ctr Res Biodivers, Extract Lab, Av Paulista 900, Sao Paulo - Brazil

^[4] Univ Paulista, Ctr Res Biodivers, Bot Lab, Sao Paulo - Brazil

^[5] Univ Paulista, Ctr Res Biodivers, Herbarium UNIP, Sao Paulo - Brazil

• Document type: Journal article
• Source: PHARMACOGNOSY MAGAZINE; v. 15, n. 60, p. 1-11, JAN-MAR 2019.
• Web of Science Citations: 0

Abstract

Background: It is estimated that in Brazil 59,700 new cases of breast and 68,220 new cases of prostate cancer will occur, in 2018, in Brazil. For that reason, there is a need for introducing new antitumor drugs in therapy. Objective: The objective of the study is to bioguide fractionate the organic/aqueous extracts from the aerial organs of Picrolemma sprucei (EB1151 and EB1152) aiming the identification of antitumor compounds and to evaluate the influence of EB1151 on mice behavioral phenotype. Materials and Methods: The breast (MCF-7) and prostate (PC-3) cancer cell lines cytotoxicity assay were assessed using the sulforhodamine B method. Fractionation was carried out by thin layer and column chromatographic techniques. The influence of the intraperitoneal administration of nonlethal dose (NLD, 39.1 mg/kg) of EB1151 to Balb-c male mice was assessed in open cage and open-field (OF) apparatuses. Results: FCHCl3partition phases from EB1151 and EB1152 showed cytotoxic activity against both cancer cells, as did fractions UNIP343, which contains sergeolide and isobruceine B, and UNIP344, which contains sergeolide and 15-deacetylsergeolide. Higher doses of EB1151 showed significant alterations in response to touch, hindquarter fall, irritability, auricular and corneal reflexes, ataxia, micturition, ptosis, piloerection, hypothermia, and cyanosis, and NLD showed alterations in general activity and response to touch, but recovery was obtained up to the end of the experiment. Conclusions: Quassinosides from the aerial organs of P. sprucei showed significant cytotoxicity against MCF-7 and PC-3 cell lines. Extract EB1151 has impaired general activity and activity in the OF apparatus, but recovery was observed. Abbreviations used: IBAMA/CGen/MMA: Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renovaveis/Conselho de Gestao do Patrimnio Genetico/Ministerio do Meio Ambiente; UNIP: Universidade Paulista; g: Gram; h: Hour; mL: Milliliters; FCHCl3: Fraction chloroform; FBuOH: Fraction butanol; FH2O: Fraction water; FHEX: Fraction hexane; FDCM: Fraction dichloromethane; FMeOH: Fraction methanol; FACN15: Fraction acetonitrile 15%; FACN50: Fraction acetonitrile 50%; FAcEt: Fraction ethyl acetate; CDCl3: Deuterated chloroform; NH4OH: Amonium hydroxide; DOXO: Doxorubicin; M: Molar; TCA: Trichloroacetic acid; mg: Microgram; IC50: 50% inhibitory concentration; CEUA/ICS/UNIP: Comissao de tica no Uso de Animais/Instituto de Ciencias da Sade/Universidade Paulista; kg: cm(3): Cubic centimeters; Kilogram; BP: Behavioral phenotype; OF: Open-field apparatus; NLD: Nonlethal dose; mL: Microliters; ANOVA: Analysis of variance; FAkl: Fraction alkaloids; mM: Millimolar; DNA: Desoxyribonucleic acid. (AU)