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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Screening protein-ligand interactions using ¹H NMR techniques for detecting the ligand

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Author(s):
Isis Martins Figueiredo [1] ; Anita Jocelyne Marsaioli [2]
Total Authors: 2
Affiliation:
[1] Universidade Estadual de Campinas. Instituto de Química. Departamento de Química Orgânica - Brasil
[2] Universidade Estadual de Campinas. Instituto de Química. Departamento de Química Orgânica - Brasil
Total Affiliations: 2
Document type: Journal article
Source: Química Nova; v. 30, n. 7, p. 1597-1605, 2007-00-00.
Abstract

NMR is a valuable screening tool for the binding of ligands to proteins providing structural information on both protein and ligands and is thus largely applied to drug-discovery. Among the recent NMR techniques to probe weak binding protein-ligand complexes we have critically evaluated the advantages and disadvantages of STD (Saturation Transfer Difference), WaterLOGSY (Water Ligand Observation with Gradient Spectroscopy), NOE pumping and DOSY-NOESY (Diffusion-Ordered NOESY) using a mixture of BSA (bovine serum albumin) plus salicylic acid, caffeine, citric acid, adipic acid and D-glucose. (AU)