Measurement of glucocorticoid and progesterone metabolites in feces of blue fronted parrot (Amazona aestiva)

Author(s)

Caroline Junko Fujihara ^[1] ; Wolff Camargo Marques Filho ^[2] ; Ana Livia Rocha Monteiro ^[3] ; Rodrigo Freitas Bittencourt ^[4] ; Carla Martins Queiroz ^[5] ; Ricardo José Garcia Pereira ^[6] ; João Carlos Pinheiro Ferreira ^[7]

Affiliation

^[1] Unesp. Faculdade de Medicina Veterinária e Zootecnia - Brasil

^[2] Unesp. Faculdade de Medicina Veterinária e Zootecnia - Brasil

^[3] Fiscal Estadual Agropecuário da Agência de Defesa Agropecuária do Estado do Ceará - Brasil

^[4] Universidade Federal da Bahia - Brasil

^[5] Unesp. Faculdade de Medicina Veterinária e Zootecnia - Brasil

^[6] USP. Faculdade de Medicina Veterinária e Zootecnia - Brasil

^[7] UnesP. Faculdade de Medicina Veterinária e Zootecnia - Brasil

• Document type: Journal article
• Source: Ciênc. anim. bras.; v. 15, n. 3, p. 277-288, 2014-09-00.

Abstract

The objectives of the present study were to evaluate fecal concentrations of metabolites of glucocorticoids, measured by enzyme immunoassay with a cortisol antibody and by radioimmunoassay with a corticosterone antibody, and progesterone by radioimmunoassay with a progesterone antibody in blue-fronted parrot (Amazona aestiva) after ACTH challenge.The adrenal stimulation with ACTH (25 UI/animal) resulted in an increase of fecal glucocorticoids metabolites concentration, but it did not affect the concentrations of fecal progesterone metabolites. Although there were no synchronized peaks of glucocorticoid metabolites excretion measured by enzyme immunoassay and radioimmunoassay, there were two peaks of excretion, one at 2-4 hours and other at 8-10 hours. Despite the occurrence of peaks, the analysis of fecal glucocorticoids metabolites and progesterone metabolites showed no effect of group (control and treatment), moment (hours of sampling) and sex. (AU)

• FAPESP's process: 09/05864-8 - Physiologic validation of glucocorticoids metabolites measurement in Blue fronted Parrot feces
• Grantee: João Carlos Pinheiro Ferreira
• Support Opportunities: Regular Research Grants