| Full text | |
| Author(s): |
Renato Mamede de Castro Montini
[1]
;
José Raimundo de Souza Passos
[2]
;
Augusto Ferreira da Eira
[3]
Total Authors: 3
|
| Affiliation: | [1] Universidade Estadual Paulista. Faculdade de Ciências Agronômicas. Departamento de Produção Vegetal - Brasil
[2] Universidade Estadual Paulista. Instituto de Biociências. Departamento de Bioestatística - Brasil
[3] Universidade Estadual Paulista. Faculdade de Ciências Agronômicas. Departamento de Produção Vegetal - Brasil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Brazilian Journal of Microbiology; v. 37, n. 1, p. 90-95, 2006-03-00. |
| Abstract | |
The mycelium growth kinetics and vigor of shiitake (Lentinula edodes (Berk.) Pegler) strains LE 96/17, LE 98/51, LE 98/53, and LE 98/56 were studied under different agar medium compositions. The strains were from the mycological collection of the Módulo de Cogumelos, Faculdade de Ciências Agronômicas, Unesp-Botucatu, Brazil. Mycelium fragments from stock cultures were transferred to Petri dishes with Sawdust extract-Dextrose-Agar medium. The area of growth and vigor (density) of the mycelia were daily recorded with a digital camera, during incubation, until the complete colonization of the Petri dish. The images were analyzed by the freeware UTHSCSA ImageTool, v. 2.0, developed by the University of Texas Health Science Center, San Antonio. The kinetics of mycelium growth, as measured by the mycelium area (mm²), has as a deterministic component an exponential function of Gompertz. The vigor, as evaluated by mycelium color in gray scale, was similar for all strains, reached a maximal value between the 4th and 5th day of incubation and decreased further on. The velocity of growth of L. edodes strains was lower in enriched culture media, while vigor was higher. Digital monitoring permits a objective evaluation of the growth kinetics of L. edodes in vitro. (AU) | |
| FAPESP's process: | 98/07726-5 - Edible and medicinal mushrooms: cultivation technology, biochemical characterisation and protection effects of Agaricus blazei Murrill (sun mushroom) and Lentinula edodes (Berk.) Pegler (shiitake) mushrooms |
| Grantee: | Augusto Ferreira da Eira |
| Support Opportunities: | Research Projects - Thematic Grants |