| Full text | |
| Author(s): |
Vânia Tonetto-Fernandes
;
Luciane M. Ribeiro-Neto
[2]
;
Ieda T.N. Verreschi
[3]
;
Jean Fiet
[4]
;
José Gilberto H. Vieira
[5]
;
Claudio E. Kater
[6]
Total Authors: 6
|
| Document type: | Journal article |
| Source: | Arquivos Brasileiros de Endocrinologia e Metabologia; v. 47, n. 2, p. 171-176, 2003-04-00. |
| Abstract | |
Serum 21-deoxycortisol (21DF) has been considered a useful hormonal marker for the diagnosis of congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency (21OHD). Although several radioimmunoassay (RIA) methods for 21DF have been reported, none are commercially available. We developed a RIA adapted for 21DF determination, preceded by ether-extraction and liquid chromatographic separation (HPLC) of samples. The assay was employed to evaluate children (15F/10M) with the classic form of 21OHD and a control group (5F/8M). The antibody obtained, in addition to efficient HPLC separation, permitted tritiated cortisol instead of 21DF to be used, since labeled ³H-21DF is expensive and difficult to obtain. Serum cortisol levels were reduced in patients with 21OHD (48h following therapy withdrawal) as compared to controls (2.1±2.1 vs. 16.2±7.0mug/dl), whereas serum levels of 21DF were significantly elevated (1,359±853ng/dl, ranging from 434 to 3,079) in the former, but consistently below the sensitivity limit of the assay (156ng/dl) in the latter group. The reported method, although devoid of sensitivity for its application in normal subjects, permits 21DF to be quantified in patients with 21OHD, with adequate sensitivity and specificity to diagnose and follow patients with this condition. (AU) | |