Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Transient gene expression in electroporated intact tissues of Stylosanthes guianensis (Aubl.) Sw.

Full text
Author(s):
Vera Maria Quecini [1] ; Maria Lúcia Carneiro Vieira [2]
Total Authors: 2
Affiliation:
[1] Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
[2] Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz. Departamento de Genética
Total Affiliations: 2
Document type: Journal article
Source: Scientia Agricola; v. 58, n. 4, p. 759-765, 2001-12-00.
Abstract

Genetic transformation though protoplast electroporation has been established for commercially important plant species. In this work, explant sources, electric field strengths, electroporation buffers, DNA forms and osmotic pretreatment were assayed in order to optimize transient reporter gene expression in electroporated tissues of Stylosanthes guianensis, a tropical forage legume. Higher transformation rates were obtained employing cotyledonary explants and an electric field strength of 250 V cm-1. Linear plasmid DNA, chloridefree electroporation buffer and osmotic pretreatment with 1.6 mol L-1 mannitol also improved transient transformation but non-significantly. Transgene specific PCR amplification was employed to prove the transformed status of the tissues. (AU)