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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs

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Author(s):
DL Ambrósio [1] ; CF Barbosa [2] ; VF Vianna [3] ; RMB Cicarelli [4]
Total Authors: 4
Affiliation:
[1] Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Ciências Biológicas - Brasil
[2] Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Ciências Biológicas - Brasil
[3] Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Ciências Biológicas - Brasil
[4] Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Ciências Biológicas - Brasil
Total Affiliations: 4
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 99, n. 6, p. 617-620, 2004-10-00.
Abstract

Pre-mRNA maturation in trypanosomatids occurs through a process called trans-splicing which involves excision of introns and union of exons in two independent transcripts. For the first time, we present the standardization of Trypanosoma cruzi permeable cells (Y strain) as a model for trans-splicing study of mRNAs in trypanosomes, following by RNase protection reaction, which localizes the SL exon and intron. This trans-splicing reaction in vitro was also used to analyze the influence of NFOH-121, a nitrofurazone-derivative, on this mechanism. The results suggested that the prodrug affects the RNA processing in these parasites, but the trans-splicing reaction still occurred. (AU)