| Full text | |
| Author(s): |
Guilherme Silva Julian
[1]
;
Renato Watanabe de Oliveira
[2]
;
Sergio Tufik
[3]
;
Jair Ribeiro Chagas
Total Authors: 4
|
| Affiliation: | [1] Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Psicobiologia - Brasil
[2] Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Psicobiologia - Brasil
[3] Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Psicobiologia - Brasil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Jornal Brasileiro de Pneumologia; v. 42, n. 3, p. 211-214, 2016-06-00. |
| Abstract | |
ABSTRACT Obstructive sleep apnea (OSA) has been associated with oxidative stress and various cardiovascular consequences, such as increased cardiovascular disease risk. Quantitative real-time PCR is frequently employed to assess changes in gene expression in experimental models. In this study, we analyzed the effects of chronic intermittent hypoxia (an experimental model of OSA) on housekeeping gene expression in the left cardiac ventricle of rats. Analyses via four different approaches-use of the geNorm, BestKeeper, and NormFinder algorithms; and 2−ΔCt (threshold cycle) data analysis-produced similar results: all genes were found to be suitable for use, glyceraldehyde-3-phosphate dehydrogenase and 18S being classified as the most and the least stable, respectively. The use of more than one housekeeping gene is strongly advised. (AU) | |
| FAPESP's process: | 11/16011-6 - Evaluation of the effect of hypoxia on the expression and activity of neprilysin (EC 3.4.24.11), a protease involved in Alzheimer's Disease |
| Grantee: | Renato Watanabe de Oliveira |
| Support Opportunities: | Scholarships in Brazil - Master |