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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection

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Author(s):
Cecília Luiza Simões dos Santos [1] ; Mariana Aparecida Antunes Bastos [2] ; Maria Anice Mureb Sallum [3] ; Iray Maria Rocco [4]
Total Authors: 4
Affiliation:
[1] Instituto Adolfo Lutz. Serviço de Virologia - Brasil
[2] Instituto Adolfo Lutz. Serviço de Virologia - Brasil
[3] Universidade de São Paulo. Faculdade de Saúde Pública. Departamento de Epidemiologia - Brasil
[4] Instituto Adolfo Lutz. Serviço de Virologia - Brasil
Total Affiliations: 4
Document type: Journal article
Source: Revista do Instituto de Medicina Tropical de São Paulo; v. 45, n. 1, p. 11-16, 2003-01-00.
Abstract

In 2001, an autochthonous case of dual viremia, resulting from naturally acquired dengue virus DEN-1 and DEN-2 infections was detected during the dengue outbreak that occurred in Barretos, a city with about 105,000 inhabitants in the North region of São Paulo State. Serotype identification was based on virus isolation to C6/36 mosquito cells culture and immunofluorescence assays using type-specific monoclonal antibodies. The double infection was also confirmed by reverse transcriptase polymerase chain reaction (RT-PCR). Comparative analysis of the 240-nucleotide sequences of E/NS1 gene junction region between the genome of DEN-1 and DEN-2 isolates of the corresponding reference Nauru and PR 159S1 strains, respectively, showed some nucleotide differences, mainly silent mutations in the third codon position. Results of maximum likelihood phylogenetic analysis of E/NS1 gene sequences indicated that both genotypes of DEN-1 and DEN-2 viruses recovered from double infection in Barretos belonged to genotypes I and III, respectively. (AU)