The Influence of Light Exposure in Ambiance during Pregnancy in Maternal and Fetal Outcomes: An Experimental Study

Author(s)

Sarri, Vitor Coca ^[1] ; Ferrari, Beatriz Maria ^[1] ; Magalhaes, Larissa Fernandes ^[2] ; Rodrigues, Paula Almeida ^[1] ; Rezende, Almir Coelho ^[3] ; Andrade Brunherotti, Marisa Afonso ^[4]

Affiliation

^[1] Univ Franca, Dept Med, Franca, SP - Brazil

^[2] Univ Franca, Dept Vet Med, Franca, SP - Brazil

^[3] Univ Franca, Dept Phys Therapy, Franca, SP - Brazil

^[4] Univ Franca, Dept Hlth Promot & Med, Ave Doutor Armando de Sales Oliveira 201, BR-14404600 Franca, SP - Brazil

• Document type: Journal article
• Source: Revista Brasileira de Ginecologia e Obstetrícia; v. 41, n. 1, p. 24-30, JAN 2019.
• Web of Science Citations: 0

Abstract

Objective The aim of this study is to evaluate whether exposure to different environmental lighting conditions affects the reproductive parameters of pregnant mice and the development of their offspring. Methods Fifteen pregnant albino mice were divided into three groups: light/dark, light, and dark. The animals were euthanized on day 18 of pregnancy following the Brazilian Good Practice Guide for Euthanasia of Animals. Maternal and fetal specimens were measured and collected for histological evaluation. Analysis of variance (ANOVA) test was used for comparison of the groups considering p 0.05 to be statistically significant. Results There was no significant difference in the maternal variables between the three groups. Regarding fetal variables, significant differences were observed in the anthropometric measures between the groups exposed to different environmental lighting conditions, with the highest mean values in the light group. The histological evaluation showed the same structural pattern of the placenta in all groups, which was within the normal range. However, evaluation of the uterus revealed a discrete to moderate number of endometrial glands in the light/dark and light groups, which were poorly developed in most animals. In the fetuses, pulmonary analysis revealed morphological features consistent with the transition from the canalicular to the saccular phase in all groups. Conclusion Exposure to different environmental lighting conditions had no influence on the reproductive parameters of female mice, while the offspring of mothers exposed to light for 24 hours exhibited better morphometric features. Resumo Objetivo Avaliar se a exposicAo a diferentes luminosidades no ambiente afeta parametros reprodutivos de camundongos femeas prenhas e o desenvolvimento de sua prole. Metodos Foram selecionados para o estudo 15 camundongos femeas albinas prenhas. Os camundongos foram separados em grupos: luz/escuro, luz e escuro. As femeas foram eutanasiadas no 18 degrees dia de gestacAo, seguindo as recomendacoes do Guia Brasileiro de Boas Praticas para a Eutanasia de animais. Tanto pecas maternas como fetais foram mensuradas e coletadas para avaliacAo histologica. Foi utilizado o teste de Analise de variantes (Anova) para comparacAo dos grupos, considerando estatisticamente significativo o valor de p 0,05. Resultados Ao comparar as variaveis maternas entre os tres grupos, nAo foi encontrada diferenca estatistica significativa. Em relacAo as variaveis fetais, houve diferencas estatisticas entre as medidas de antropometria dos grupos submetidos a diferentes luminosidades do ambiente, com melhores valores medios no grupo luz. Histologicamente, a avaliacAo placentaria evidenciou em todos os grupos o mesmo padrAo estrutural, com todos dentro da normalidade. No entanto, a avaliacAo de uteros, tanto do grupo luz/escuro quanto do grupo luz, mostrou quantidade discreta a moderada de glandulas endometriais, com pouco desenvolvimento na maioria dos animais. Nos fetos, analise pulmonar evidenciou caracteristicas morfologicas compativeis com a transicAo da fase canalicular para sacular em todos os grupos. ConclusAo As exposicoes a diferentes luminosidades no ambiente nAo influenciaram nos parametros reprodutivos das femeas, entretanto, a ninhada das mAes que receberam luz em todo periodo apresentou melhores medidas morfometricas. (AU)

• FAPESP's process: 15/17748-3 - Environment light influence on the pregnancy period and impact in reproductive parameters: experimental study in mice
• Grantee: Vitor Coca Sarri
• Support Opportunities: Scholarships in Brazil - Scientific Initiation