| Full text | |
| Author(s): Show less - |
Rosa, Marcela Nunes
[1]
;
Evangelista, Adriane Feijo
[1]
;
Leal, Leticia Ferro
[1]
;
De Oliveira, Cristina Mendes
[1]
;
Oliveira Silva, Viviane Aline
[1]
;
Munari, Carla Carolina
[1]
;
Munari, Fernanda Franco
[1]
;
Matsushita, Graziela De Macedo
[2]
;
Dos Reis, Ricardo
[3]
;
Andrade, Carlos Eduardo
[3]
;
Souza, Cristiano de Padua
[3]
;
Reis, Rui Manuel
[4, 1, 5]
Total Authors: 12
|
| Affiliation: | [1] Barretos Canc Hosp, Mol Oncol Res Ctr, Barretos, SP - Brazil
[2] Barretos Canc Hosp, Dept Pathol, Barretos, SP - Brazil
[3] Barretos Canc Hosp, Dept Gynecol Oncol, Barretos, SP - Brazil
[4] Univ Minho, Sch Hlth Sci, Life & Hlth Sci Res Inst ICVS, Braga - Portugal
[5] 3Bs PT Govt Associate Lab, Braga - Portugal
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 9, FEB 13 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
Cervical cancer is the fourth most common cancer in women. Although cure rates are high for early stage disease, clinical outcomes for advanced, metastatic, or recurrent disease remain poor. To change this panorama, a deeper understanding of cervical cancer biology and novel study models are needed. Immortalized human cancer cell lines such as HeLa constitute crucial scientific tools, but there are few other cervical cancer cell lines available, limiting our understanding of a disease known for its molecular heterogeneity. This study aimed to establish novel cervical cancer cell lines derived from Brazilian patients. We successfully established one (HCB-514) out of 35 cervical tumors biopsied. We confirmed the phenotype of HCB-514 by verifying its' epithelial and tumor origin through cytokeratins, EpCAM and p16 staining. It was also HPV-16 positive. Whole-exome sequencing (WES) showed relevant somatic mutations in several genes including BRCA2, TGFBR1 and IRX2. A copy number variation (CNV) analysis by nanostring and WES revealed amplification of genes mainly related to kinases proteins involved in proliferation, migration and cell differentiation, such as EGFR, PIK3CA, and MAPK7. Overexpression of EGFR was confirmed by phospho RTK-array and validated by western blot analysis. Furthermore, the HCB-514 cell line was sensitive to cisplatin. In summary, this novel Brazilian cervical cancer cell line exhibits relevant key molecular features and constitutes a new biological model for pre-clinical studies. (AU) | |
| FAPESP's process: | 15/20077-3 - High-risk HPV DNA detection and analysis of the mutational status of KRAS and PIK3CA genes and inflammatory cytokines panel in patients with esophageal cancer and megaesophagus with and without cancer |
| Grantee: | Fernanda Franco Munari |
| Support Opportunities: | Scholarships in Brazil - Master |