Nitrate and ammonium proportion plays a key role in copper phytoextraction, improving the antioxidant defense in Tanzania guinea grass

Author(s)

de Souza Junior, Joao Cardoso ^[1] ; Nogueirol, Roberta Correa ^[2] ; Monteiro, Francisco Antonio ^[1]

Affiliation

^[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Soil Sci Dept, Padua Dias 11, BR-13418900 Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Crop Sci Dept, Padua Dias 11, BR-13418900 Piracicaba, SP - Brazil

• Document type: Journal article
• Source: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 171, p. 823-832, APR 30 2019.
• Web of Science Citations: 1

Abstract

Various nitrate and ammonium proportions (NO3-/NH4+) in the growth media can increase metal phytoextraction compared to supplying solely NO, However, there are no studies showing these effects in plants under copper (Cu) contamination as well as their consequences in plant stress tolerance. The objective was to evaluate the effect of NO3-/NH4+ proportions in Cu phytoextraction by Panicum maximum cv. Tanzania and its consequence in the oxidative stress, photosynthesis, and antioxidant system under Cu stress. The experiment was carried out in a randomized complete block design, by using a 3 x 4 factorial with six replications. Three NO3-/NH4+ proportions (100/0, 70/30, and 50/50) were combined with four Cu rates (0.3, 250, 500, and 1000 mu mol L-1) in the nutrient solution. It was found that the largest Cu accumulation in the shoots occurred at the first harvest of the plants supplied with 70/30 NO3-/NH4+ and Cu 1000 mu mol L-1. Such plants also displayed high concentrations of proline in the shoots as well as high superoxide dismutase activity in the roots. Malondialdehyde concentration was high in the plant parts at the Cu rate of 1000 mu mol L-1. Hence, transpiration rates, stomatal conductance, quantum efficiency of photosystem II, electron transport rate, and net photosynthesis were all low at the Cu rate of 1000 mu mol L-1. Catalase, guaiacol peroxidase, ascorbate peroxidase, and glutathione reductase activities in the roots were high when plants were exposed to Cu 1000 mu mol L-1. In conclusion, the combination of NO3- with NH4+ increases copper phytoextraction that causes oxidative stress, but also favors the antioxidant system of Tanzania guinea grass in attempt to tolerate such stress. (AU)

• FAPESP's process: 15/20624-4 - Plant mineral nutrition and Tanzania guinea grass tolerance to stress caused by metals
• Grantee: Francisco Antonio Monteiro
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/20625-0 - Nitrate/ammonium relationship and Tanzania guinea grass tolerance to stress caused by copper
• Grantee: João Cardoso de Souza Junior
• Support Opportunities: Scholarships in Brazil - Master