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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Modulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis

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Author(s):
Caleffi-Ferracioli, Katiany R. [1] ; Cardoso, Rosilene F. [1] ; de Souza, Joao V. P. [1] ; Murase, Leticia S. [1] ; Canezin, Pedro H. [1] ; Scodro, Regiane B. L. [1] ; Siqueira, Vera L. D. [1] ; Pavan, Fernando R. [2]
Total Authors: 8
Affiliation:
[1] Univ Estadual Maringa, Dept Clin Anal & Biomed, Lab Med Bacteriol, Maringa, Parana - Brazil
[2] Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Biol Sci, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: FUTURE MICROBIOLOGY; v. 14, n. 3, p. 185-194, FEB 2019.
Web of Science Citations: 1
Abstract

Aim: To evaluate modulatory effect of verapamil (VP) in rifampicin (RIF) activity and its effect in efflux pumps (EPs) transcript levels in Mycobacterium tuberculosis. Materials \& methods: RIF and VP minimal inhibitory concentration, combinatory effect and detection of mutations were determined in 16 isolates. EPs transcript levels were determined in four isolates by real-time PCR after exposure to drugs. Results: VP showed good combinatory effect among RIF-resistant isolates. This effect was also observed in the relative transcript levels of EPs, mainly after 72h of exposure, depending on the EP gene, genotype and the resistance profile of the isolate. Conclusion: Additional regulatory mechanisms in the EP activities, as well as, interactions with other drug-specific resistance mechanisms need further investigation in M. tuberculosis. (AU)

FAPESP's process: 13/14957-5 - Research potential against tuberculosis of a new class of furoxan compounds and nanostructured compounds of the ruthenium(II) and copper (II)
Grantee:Fernando Rogério Pavan
Support Opportunities: Research Grants - Young Investigators Grants