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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Structure-function relationship of a citrus salicylate methylesterase and role of salicylic acid in citrus canker resistance

Full text
Author(s):
de Lima Silva, Caio Cesar [1] ; Shimo, Hugo Massayoshi [1] ; de Felicio, Rafael [1] ; Mercaldi, Gustavo Fernando [1] ; Rocco, Silvana Aparecida [1] ; Benedetti, Celso Eduardo [1]
Total Authors: 6
Affiliation:
[1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, BR-13083100 Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, MAR 7 2019.
Web of Science Citations: 0
Abstract

Salicylic acid (SA) and its methyl ester, methyl salicylate (MeSA) are well known inducers of local and systemic plant defense responses, respectively. MeSA is a major mediator of systemic acquired resistance (SAR) and its conversion back into SA is thought to be required for SAR. In many plant species, conversion of MeSA into SA is mediated by MeSA esterases of the SABP2 family. Here we show that the Citrus sinensis SABP2 homologue protein CsMES1 catalyzes the hydrolysis of MeSA into SA. Molecular modeling studies suggest that CsMES1 shares the same structure and SA-binding mode with tobacco SABP2. However, an amino acid polymorphism in the active site of CsMES1-related proteins suggested an important role in enzyme regulation. We present evidence that the side chain of this polymorphic residue directly influences enzyme activity and SA binding affinity in CsMES proteins. We also show that SA and CsMES1 transcripts preferentially accumulate during the incompatible interaction between Xanthomonas aurantifolii pathotype C and sweet orange plants. Moreover, we demonstrate that SA and MeSA inhibited citrus canker caused by Xanthomonas citri, whereas an inhibitor of CsMES1 enhanced canker formation, suggesting that CsMES1 and SA play a role in the local defense against citrus canker bacteria. (AU)

FAPESP's process: 11/20468-1 - Molecular mechanisms involved in pathogen adaptation and virulence, host resistance and symptom development in citrus-bacteria interactions
Grantee:Celso Eduardo Benedetti
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/22507-0 - Functional characterization of CsWRKY, a sweet orange (citrus sinensis) transcription factor involved in citrus canker resistance
Grantee:Hugo Massayoshi Shimo
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/03151-8 - Aminoacil-tRNA Synthetases as Targets for Development of Agrobactericides intented for Control of Plant Diseases caused by Xanthomonas species
Grantee:Gustavo Fernando Mercaldi
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/22399-2 - Biochemical and functional analysis of a citrus sinensis protein, CsEIE, involved in the defense against Xanthomonas citri
Grantee:Caio Cesar de Lima Silva
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)